β-连环蛋白在人毛囊发育形成中的表达

Expression of β-catenin in the development of human hair follicle

目的 :研究 β -连环蛋白 ( β -catenin)在人毛囊从胚胎到成熟发育过程中的表达及意义。 方法 :采用SP免疫组化技术(IHC)对不同胚胎龄及成年期的正常人头皮标本进行染色 ,分析比较不同时期毛囊 β -catenin表达及其差异。 结果 :β -catenin蛋白的表达与毛囊发育的不同阶段具有相关性 ,早期形成的毛芽中未见 β -catenin表达 ,而胚胎毛囊发育后期的外根鞘及膨突区中出现β -catenin表达 ,在成人发育成熟的毛囊中 ,β -catenin表达明显增强 ,阳性细胞集中于毛囊外根鞘内侧。结论 :β -catenin在毛囊不同发育阶段表达与分布的差异 ,提示 β

Objective:To study the expression and significance of β-catenin in the development of human hair follicle. Methods:]A total of 12 frozen tissues of skin from normal embryo and adult were stained by SP immunohistochemical method(IHC) with specific anti-β-catenin antibody. The expression of β-catenin in different phages of human hair follicles was analyzed.Results:Expression of β-catenin protein was significantly increased in the development period of human hair follicles, and it showed great correlation to the different phages. Expression of β-catenin was not detected in hair bud of early embryo skin, while appeared in outer root sheath and bulge of hair follicles in later development stage. And the strong expression was observed in adult skin tissues, which concentrated in the inside of outer root sheath. Conclusion:The expression and distribution of β-catenin was obviously distinct in the process of development, suggesting a possible role of β-catenin in the formation and development of human hair follicles.