HSP_(70BCG)-Daudi肿瘤肽复合物对γδT细胞的活化作用

The Cytolytic Effects of HSP_(70BCG)-Daudi Antigen Complexes on Human γδT Lymphocytes

用固相抗体法[1] 体外扩增人PBMC的γδT细胞 ,静息化处理后用HSP70BCG Daudi肿瘤肽复合物再次刺激研究HSP70BCG Daudi肿瘤肽复合物对γδT细胞的活化作用。MTT检测γδT细胞的细胞毒作用及TNF α生物学活性。3 H TdR掺入法测定细胞增殖。HSP70BCG Daudi肿瘤肽复合物活化的γδT细胞在效靶比为 10∶1及 5∶1时对靶细胞Daudi的杀伤活性分别为 88 36 %±8 2 1%及 75 2 3%± 16 36 % ,与对照组有显著性差别。 2 4及 4 8h增殖结果各组间无差别 ;未检测出各刺激组的上清中TNF α生物学活性有何差别。结论表明HSP70BCG

PBMC were culured with anti-TCRγδ antibody,and then restimulated with HSP 70BCG-Daudi antigen complexes study to the cytolytic effects of HSP 70BCG-Daudi antigen complexes on human γδT lymphocytes. The cytotoxicity activity of γδT cells against Daudi and the level of TNF-α were determined by using MTT calorimetric assay. 3H-TdR incorporation was used to analyze the proliferation of γδT cells. When the ratio of E/T was 10∶1,the cytotoxicity of γδT cells against Daudi was 88.36%±8.21%;While the ratio of E/T was 5∶1,the cytotoxicity of γδT cells against Daudi was 75.23%±16.36%. The 24 and 48 hours proliferation among each group had no difference. There was no difference in the bioactivity of TNF-α among each group. These results showed that the γδT cells exhibited potent cytotoxicity against Daudi when they were restimulated with HSP 70BCG-Daudi antigen complexes.