重组人肿瘤坏死因子-α诱导黏液表皮样癌细胞凋亡

Apoptosis of mucoepidermoid carcinoma cells induced by recombinant human tumor necrosis factor-α

目的 :探讨重组人肿瘤坏死因子 α(rhTNF α)作用于人黏液表皮样癌细胞 (MEC 1 )的效应与机制 .方法 :应用细胞记数、DNA降解片段琼脂糖凝胶电泳观察、电镜观察和细胞周期测定rhTNF α作用后MEC 1细胞的凋亡状况 .结果 :rhTNF α作用于MEC 1细胞后 ,导致细胞存活数目减少 ,大量细胞发生凋亡 ;基因组DNA分解成寡聚核苷酸片段 ,DNA琼脂糖凝胶电泳有梯形条带 ;细胞周期发生改变 ,形态学观察细胞核皱缩 ,染色质边集 .结论 :rhTNF α是通过MEC

AIM: To explore the apoptosis mechanism of mucoepidermoid carcinoma cells (MEC 1) induced by recombinant human tumor necrosis factor α (rhTNF α). METHODS: By cell counting, the qualitative analysis of DNA fragmentation, the assays of the morphological changes and the cell cycle change, we evaluated the growth condition and apoptosis induction of the MEC 1 cells treated with rhTNF α. RESULTS: Treated with rhTNF α, the living MEC 1 cell numbers decreased and massive of apoptotic cells occurred, DNA fragmented into nucleosemal oligomers,agarose gel eletrophoresis of fragmented DNA showed a ladder like pattern; the cell cycle changed and electron microscopic observation showed chromatin aggregating beneath the nuclear envelop, nuclear contraction. CONCLUSION: rhTNF α exerts its cytotoxic effects on the MEC 1 through inducing the cells apoptosis.