多发性硬化患者造血干/祖细胞及其亚群富集纯化的研究

Collection and purification of CD34^+ hematopoietic stem (progenitor) cells and its sub-groups from patients with multiple sclerosis

目的 :获得高纯度造血干 /祖细胞 (HSC/HPC)及其亚群 ,用于多发性硬化 (MS)造血干细胞移植治疗和生物学特性的研究 .方法 :用免疫磁珠法 (MiniMACS)从MS患者骨髓中富集CD34+ 细胞后再用荧光激活细胞分选 (fluorescentacti vatedcellsorter,FACS)纯化CD34+ /CD38+ 和CD34+ /CD38-细胞亚群 .结果 :MiniMACS分选的CD34+ 细胞群纯度达 91 % ,FACS分选的CD34+ /CD38-和CD34+ /CD38+ 两细胞亚群纯度可达 98%以上 .结论 :MiniMACS和FACS两者结合可迅速大量纯化HSC/HPC及其重要细胞亚群 ,可用于临床MS造血干细胞移植。

AIM: To collect and purify the bone marrow CD34 + hematopoietic stem cells and its sub groups from the patients with multiple sclerosis. METHODS: CD34 + cells were collected and purified by means of imunomagnetic beads on MiniMACS device. Flow cytometry was emplyed to determine percentage of CD34 + cells, CD34 +/CD38 - cells and CD34 +/CD38 + cells before and after miniMACS. RESULTS: The purity of CD34 + cells amounted to more than 91% after the purification. The purity of CD34 +/CD38 - cells and CD34 +/CD38 + cells amounted to more than 98% after fluorescent activated cell sorter (FACS). CONCLUSION: The combination of MiniMACS and FACS can sort effectively the bone marrow CD34 + cells and its sub groups so that they may be used for hematopoietic stem cell transplantation, gene therapy and biological study of hematopoietic stem cells/hematopoietic progenitor cells (HSC/HPC) and its sub groups.