脯氨酰-4-羟化酶基因在妊高征胎盘中的表达及其临床意义

Expression of prolyl-4-hydroxylase gene in placenta of patients with pregnancy induced hypertension and its clinical significance

目的 :通过检测脯氨酰 4 羟化酶 (P4H)基因在妊娠高血压综合征 (妊高征 )患者胎盘中的表达 ,了解其在妊高征病理生理机制中的作用 .方法 :采用半定量RT PCR技术检测1 0例正常妊娠妇女 (对照组 )及 1 0例中、重度妊高征患者 (妊高征组 )胎盘组织P4H和 β actinmRNA水平 .结果 :P4H与 β actin积分吸光度比值在对照组和妊高征组胎盘组织中分别为 :0 .386 82± 0 .0 7931 ;0 .4 5 6 71± 0 .0 5 80 3.显示P4HmRNA在对照组和妊高征组胎盘组织中均有表达 ,妊高征组胎盘组织P4H基因的表达明显高于对照组 ,差异有显著性 (P =0 .0 37) .结论 :妊高征患者胎盘组织中P4H基因的高表达 ,可能是妊高征一系列病理生理变化的环节之一 .

AIM: To investigate the expression of prolyl 4 hydroxylase (P4H) gene in the placenta of patients with pregnancy induced hypertension (PIH). METHODS: By using semi quantitative RT PCR, the mRNA levels of PIH and β actin of the patients with PIH and of the normal pregnant woman were measured and then compared. RESULTS: The ratio of P4H to β actin in control group was 0.38682± 0.07931 , and that in PIH group was 0.45671± 0.05803 , which suggested that mRNA level of P4H in PIH group was significantly higher than that in control group. CONCLUSION: Overexpression of P4H gene might play an important role in pathogenesis and pathophysiology of PIH.