妊娠高血压综合征患者外周血CD62L和CD11B表达的意义

Significance of expression of CD62L and CD11B in pregnancy-induced hypertension patients

目的 :探讨妊娠高血压综合征 (妊高征 )患者外周血CD6 2L和CD1 1B表达的变化及其在妊高征发病中的作用 .方法 :应用流式细胞术 (flowcytometry,FCM)检测 2 5例妊高征患者和 2 4例正常孕妇 (对照组 )的外周血中CD1 1B和CD6 2L的表达 .采用酶联免疫吸附实验 (ELISA)测定可溶性L 选择素(sL selectin)的浓度 .结果 :妊高征组外周血CD6 2L的平均抗体阳性百分率 (2 2 .3± 2 .5 ) %明显低于正常对照组 (4 1 .8± 4 .1 ) % (P <0 .0 1 ) .而血浆中可溶性L 选择素的浓度在妊高征组 (1 0 70± 89)ng/mL明显高于正常孕妇 (890± 5 8)ng/mL差异具有统计学意义 (P <0 .0 1 ) .妊高征患者CD1 1B平均抗体阳性表达率 (5 5 .9± 5 .2 ) %明显高于正常对照组 (2 1 .2± 2 .2 ) %(P <0 .0 1 ) .结论 :妊高征患者CD1 1B和血浆可溶性L 选择素明显增加 ,两者可能是参与中性粒细胞活时血管内皮损伤的重要分子 。

AIM: To study the significance of expression of CD62L and CD11B in patients with pregnancy induced hypertension (PIH). METHODS: In 25 pregnant women with PIH and 24 normal pregnant women at third trimester, CD62L and CD11B on polymorphonuclear neutrophils were analyzed by flow cytometry (FCM). Enzyme linked immunosorbent assay (ELISA) was used to measure soluble L selectin (sL selectin) plasma level. RESULTS: The expression of CD62L was significantly lower in PIH patients (22.3±2.5)% in control group (41.8±4.1)%, P < 0.01 . The expression of CD11B was significantly higher in PIH patients (55.9±5.2)% than in the normal pregnant women (21.2±2.2)%, P <0.01. sL selectin plasma level was significantly higher in PIH patients (1070±89) ng/mL than in normal pregnant women (890±58) ng/mL, P < 0.01 . CONCLUSION: In PIH patients, CD11B and sL selectin are obviously elevated, which may play a significant role in stimulating leukocyte activation during PIH.