丹参和异搏定对高能冲击波致兔肾损伤的保护作用

Protective effects of salvia miltiorrhiza and verapamil on high energy shock wave induced renal damage in rabbits

目的 :探讨丹参对高能冲击波致家兔肾损伤的保护作用及机制 .方法 :将家兔 4 5只随机分为 3组 ,丹参组、异搏定组和生理盐水对照组各 1 5只 ,ESWL前 3d分别iv丹参注射液、异搏定和生理盐水 ,测定体外冲击波碎石术 (ESWL)前后不同时间血浆中内皮素 (ET 1 )和超氧化物歧化酶 (SOD)及血清中丙二醛 (MDA)变化 .结果 :对照组ESWL后血浆中ET 1和血清中MDA值较ESWL前显著升高 (PET 1 =0 .0 1 5 ,PMDA=0 .0 1 ) ,而血浆中SOD活性显著性降低 (P =0 .0 0 3) .丹参组和异搏定组ESWL后血浆中ET 1和血清MDA较ESWL前无显著性变化 ,且高峰值均显著低于对照组 ;而血浆SOD活性虽较ESWL前降低 ,但却显著高于对照组 (丹参 :P =0 .0 0 1 ;异搏定 :P =0 .0 2 2 ) .丹参组和异搏定组两组间 3个指标均无显著性差异 .结论 :丹参与异搏定一样对高能冲击波致家兔肾损伤具有保护作用 .

AIM: To study the protective effects of salvia miltiorrhiza on high energy shock wave induced renal damage in rabbits. METHODS: A total of 45 rabbits was randomly assigned to 3 groups. Control received physiological saline and the others received salvia miltiorrhiza and verapamil for 3 days before extracorporeal shock wave lithotripy (ESWL), respectively. Plasma ET 1, SOD and serum MDA were measured. RESULTS: After ESWL, there was a significant increase in plasma ET 1 ( P =0.015) and serum MDA levels ( P =0.01) and the activity of SOD in plasma decreased significantly ( P =0.003) compared with that before ESWL in control. In salvia miltiorrhiza and verapamil groups, however, plasma ET 1 and serum MDA after ESWL were not significantly different compared with the values before ESWL and were significantly lower than those of the controlled groups. Although the levels of the plasma SOD after ESWL decreased compared with the pre ESWL levels, the activity of SOD in plasma was significantly higher than that of the controlled ones (salvia miltiorrhiza: P =0.001, verapamil: P =0.022). All parameters were not significantly different between salvia miltiorrhiza and verapamil groups. CONCLUSION: Salvia miltiorrhiza and verapamil both have protective effects on shockwave induced renal injury in rabbits.