血小板衍化生长因子对人牙髓成纤维细胞DNA和胶原蛋白合成的影响

The effects of platelet-derived growth factor on stimulating DNA and collagen protein synthesis of human dental pulp fibroblasts

目的 :观察血小板衍化生长因子 (platelet-derivedgrowthfactor ,PDGF)对人牙髓成纤维细胞(humandentalpulpfibroblast,HDPF )DNA合成和胶原蛋白合成的影响。 方法 :应用3 H -TdR和3 H -脯氨酸掺入方法 ,观察PDGF对体外培养的HDPFDNA合成和胶原蛋白合成的情况。结果 :2 0～ 6 0ng/mLPDGF可明显促进HDPFDNA的合成 ,4 0ng/mL浓度使细胞DNA合成在 36h达最大值 ;对细胞的胶原蛋白合成无明显促进作用。结论 :PDGF明显促进HDPFDNA的合成 。

AIM:DNA and collagen protein synthesis of human dental pulp fibroblasts (HDPF)were determined by treating with platelet-derived growth factor(PDGF).METHODS:HDPF were cultured with PDGF and DNA and collagen protein synthesis were measured by 3H-TdR and 3H-proline incorporation.RESULTS:PDGF significantly accelerated DNA synthesis of HDPF in dose-dependent manner over a concentration range of 20 ng/mL to 60 ng/mL ,with a maximal response at a concentration of 40 ng/mL at 36 h. PDGF did not stimulate the collage protein synthesis of HDPF.CONCLUSIN:PDGF promotes DNA synthesis of HDPF dependently, and may play an important role in pulp therapy.