摘要
目的 研究胃腔内促性腺激素释放激素 (GnRH)类似物对大鼠消化道胃泌素免疫阳性细胞及血液、胃液中胃泌素含量的影响。 方法 应用免疫组织化学和酶联免疫分析 (ELISA)方法分别检测大鼠消化道内胃泌素阳性细胞的密度及血液、胃液中胃泌素的含量。 结果 胃腔注射GnRH类似物后 ,胃壁内单位面积胃泌素阳性细胞数为 19 6 0± 3 6 3,与对照组 10 30± 2 4 1相比 (P <0 0 1) ;而十二指肠阳性细胞数为 18 0 0± 2 31,与对照组 9 0 0± 2 0 5相比 (P <0 0 1)。血液中胃泌素ELISA检测的A值为 0 5 5± 0 0 83,与对照组 0 2 3± 0 0 39相比 (P <0 0 1) ;胃液中为 0 5 2± 0 0 83,与对照组 0 30± 0 0 31相比 (P <0 0 1)。 结论 外分泌的GnRH对大鼠消化道中胃泌素的合成与分泌均起显著的促进作用。
Objective To investigate the effect of GnRH-A in gastric cavity on function of gastrin clls in digestive tract of rats. Methods The immunohistochemical ABC method and enzyme-link immunoassay were used in the experiment. Results The densities of the gastrin immunoreactivity cells in the stomach and small instestin of the experiment groups were 19.60±3.63 and 18.00±2.31 respectively, those in stomach was 10.30±2.41 while in small intestin was 9.00±2.05 of control groups respectively (P<0.01). The contents of gastrin in circulation blood and gastric liquid of the experiment groups were 0.55±0.083 and 0.52±0.083 respectively, and those in circulation blood was 0.23±0.039 whlie in gastric liquid was 0.30±0.031 in control groups respectively(P<0.01).Conclusion The exocrine GnRH in the stomach cavity significantly stimulated synthesis and secretion of gastrin cell in digestive tract of rats.
出处
《解剖学报》
CAS
CSCD
北大核心
2004年第2期190-193,共4页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目 ( 3 9770 3 88)
