恒河猴骨髓间充质干细胞的生物学特性

THE BIOLOGICAL FEATURES OF RHESUS BONE MARROW MESENCHYMAL STEM CELLS

目的 探讨骨髓间充质干细胞 (MSCs)的细胞生物学特性。 方法 免疫细胞化学检测细胞ALPase、Vimentin和Laminin活性。流式细胞术测定细胞周期和细胞表面抗原。MTT法测定细胞的生长曲线。原位杂交检测细胞端粒酶活性。 结果 MSCsALPase和Laminin呈阴性 ,Vimentin呈阳性。细胞周期中G1、S、G2期的细胞所占百分比分别为 89 5 %、7 8%、7 7% ;MSCs具有独特的表现特征 ,即CD16 6、CD2 9、CD4 4、CD10 5表达呈强阳性 ,阳性率高达 96 7%以上 ,而CD34和HLA DR呈阴性表达。细胞的平均倍增时间为 32h。细胞端粒酶活性呈阳性反应。 结论 培养的细胞为未分化的骨髓间充质干细胞 ,而不是造血干细胞和成纤维细胞 ,且细胞具有较强的增殖能力。

Objective In order to explore the biological features of bone marrow mesenchymal stem cells. Methods The ALPase activity, Vimentin and Laminin were detected by immunocytochemistry. Cell cycle and surface antigenic features were analyzed by flow cytometry technique. Cell growth curve was measured by MTT test. The telomerase activity was detected by in situ hybridization. Results The ALPase activity and Laminin were negative, Vimentin was positive in the MSCs. The percentage of G1, S, and G2 of cell cycle was 89.5%,7.8%,7.7%, respectively. MSCs were positive for CD166、CD29、CD44、CD105, and negative for CD34 and HLA-DR. The average doubling time of cells was 32 hours. The telomerase activity was positive. Conclusion The cultured cells were not hemopoietic stem cells or fibroblasts, but the MSCs that had not differentiated. Furthermore, it had relatively high proliferation ability.