鸵鸟羟基磷灰石陶瓷支架负载骨髓基质细胞异位成骨性能

Ectopic osteogenesis of ostrich hydro-xyapatite ceramic loaded with bone marrow stromal cells

目的 :观察鸵鸟羟基磷灰石陶瓷支架负载骨髓基质细胞后植入裸鼠皮下的成骨性能 .方法 :获取兔髂骨松质骨骨髓基质细胞 ,体外分离、扩增、诱导后接种于鸵鸟羟基磷灰石支架 .支架 /细胞复合物植入裸鼠背部皮下 ,支架单纯植入作为对照 .植入后 3,6wk取材 ,通过大体、组织学观察评价成骨活性 .结果 :支架 /细胞复合物植入后 3wk ,以软骨为主的大量未成熟骨在材料表面及孔隙内形成 ;植入后 6wk ,更多的成熟骨形成 .在支架材料和骨组织的邻接区域见成骨细胞及破骨细胞 ,部分区域有血管和多核巨细胞分布 ,可见软骨内成骨方式 .结论 :支架 /细胞复合物显示良好的成骨活性 。

AIM: To observe the osteogenesis of ostrich hydroxyapatite ceramic loaded with bone marrow stromal cells in a nude mice model. METHODS: Bone marrow stromal cells (BMSCs) were obtained by harvesting the cancellous bone from the iliac crest of a rabbit. The BMSCs were isolated, expanded, induced in vitro and seeded in the ostrich hydroxyapatite (OHA) ceramic scaffolds. The OHA/BMSCs composites were implanted subcutaneously into the backs of nude mice. Implantation of OHA alone was used as control. At 3 and 6 weeks after implantation, the specimens were harvested and the osteogenetic activity was evaluated by gross and histological observation. RESULTS: At 3 weeks after implantation, a large amount of immature bone composed mainly of cartilaginous bone formed in the surface and pores of the ceramics. At 6 weeks after implantation, much more mature bone had formed than before. Osteoblasts and osteoclasts were seen between the scaffolds and the local bone in the transition area and blood vessels and multinucleated cells were seen in some places. The endochondral bone formation pattern was observed. CONCLUSION: The OHA/BMSCs composites have good osteogenetic activity. This study suggests that OHA ceramics can be used as a bone tissue engineering scaffold.