反义c-myc重组腺病毒载体的构建及其对大鼠气道平滑肌细胞增殖的抑制作用

Construction of antisense recombinant adenoviral vector for c-myc and its antiproliferative effects on rat airway smooth muscle cells

目的 :观察反义c myc重组腺病毒载体对大鼠气道平滑肌细胞增殖的抑制作用 .方法 :构建大鼠反义及正义c myc细菌质粒 ,并将所得细菌质粒及腺病毒E1缺失质粒导入 2 93细胞系 ,经共转染得到正义及反义重组腺病毒载体 .MTT法检测重组腺病毒载体对大鼠气道平滑肌细胞增殖的抑制作用 ,免疫组织化学染色检测重组腺病毒载体对c Myc蛋白的表达 .结果 :反义c myc重组腺病毒载体可抑制大鼠气道平滑肌细胞增殖 ,降低气道平滑肌细胞c Myc蛋白的表达 .结论 :反义c

AIM: To observe the antiproliferative effects of antisense recombinant adenoviral vector for c myc on rat airway smooth muscle cells. METHODS: Antisense and sense bacterial plasmids for c myc were constructed. Bacterial plasmids and E1 deleted adrenoviral plasmid were cotransfected into 293 cells. Recombinant adenoviral vectors were obtained after cotransfection. The antiproliferative effects were assayed by MTT and the expression of c Myc was detected by immunohistochemistry. RESULTS: The results showed that antisense recombinant adenoviral vector for c myc could inhibit rat airway smooth muscle cells proliferation. The expression of c Myc decreased after antisense recombinant adenoviral vector for c myc was transfected into cells. CONCLUSION: Recombinant adenoviral vector antisense for c myc can inhibit rat airway smooth muscle cells proliferation.