p16,EGFR在外阴营养不良和外阴鳞癌中的表达及意义

Significance and expression of p16 and EGFR in vulvar dystrophy and squamous cell carcinoma of vulva

目的 :研究p1 6 ,EGFR在外阴营养不良、外阴鳞癌组织中的表达及其相互关系 ,探讨其在外阴营养不良发生及恶变中的作用 .方法 :采用免疫组化SABC法 ,对 1 0 0例外阴营养不良和 34例外阴鳞癌标本中p1 6 ,EGFR表达水平进行检测 .结果 :与正常皮肤及其他各型营养不良组相比 ,不典型增生组织中p1 6的阳性率降低 ,EGFR的阳性率升高 ,差异均有显著意义 (P <0 .0 5 ) ,而与鳞癌组之间差异不显著 (P >0 .0 5 ) .恶变后 ,p1 6蛋白缺失及EGFR过表达主要发生在III级鳞癌 ,且p1 6阴性的鳞癌组织中EGFR阳性率明显高于p1 6阳性组 (P <0 .0 1 ) .结论 :EGFR的高表达可能与外阴营养不良的发生有关 ,而癌基因蛋白EGFR过度表达与抑癌基因蛋白p1 6的缺失在外阴营养不良的恶变过程中可能具有重要作用 .

AIM:To study the expression of p16 and EGFR in vulvar dystrophy and squamous cell carcinoma (SCC) of vulva and to analyze their contribution to the pathogenesis and malignant transformation of vulvar dystrophy. METHODS: The expression of p16 and EGFR was investigated by SABC technique of immunohistochemistry in 100 cases of various vulvar dystrophy and 34 cases of SCC of vulva. RESULTS: Atypical hyperplasia group showed a dramatic reduction in p16 protein expression and an increase in EGFR expression compared with the normal tissue and other various dystrophy ( P <0.05), but there was no significant difference between atypical hyperplasia and the group of SCC of vulva ( P >0.05). In SCC of vulva, the loss of p16 and overexpression of EGFR occurred mainly in stage III and the expression level of EGFR was much higher in p16 negative cases than that in p16 positive cases ( P <0.01). CONCLUSION: The overexpression of EGFR may be related to the pathogenesis of vulvar dystrophy. The loss of p16 and the overexpression of EGFR may play an important role in the malignant transformation of vulvar dystrophy.