牙本质磷蛋白在犬乳恒牙替换期表达的原位杂交研究

Dentin phosphoprotein mRNA expression in replacement of primary teeth with permanent teeth of dog

目的 :观察牙本质磷蛋白 (DPP)mRNA在犬乳恒牙替换期的表达 ,寻找研究牙本质形成与损伤修复的良好模型。方法 :采用原位杂交的方法 ,检测犬牙替换期恒牙胚、发育中的恒牙和乳牙DPPmRNA的表达。结果 :犬恒牙胚、发育中的恒牙和乳牙均检测到DPPmRNA的表达 ,DPPmRNA存在强弱变化。结论 :DPP在犬牙存在表达 ,并具有时空特异性。

AIM: To observe expression of dentin phosphoprotein (DPP) in replacement of primary teeth by permanent teeth of dog,to get a better animal model on teeth development and repair after injury. METHODS: DPP mRNA expression was detected with in situ hybridization.RESULTS: DPP mRNA expressed in the permanent tooth germ,erupting permanent tooth and deciduous tooth in the progress of replacement. The expression varied at different positions in the deciduous tooth. CONCLUSION:DPP was expressed in dog teeth.The expression of DPP has tempro- spacial characters.