小鼠表皮角质形成细胞中TNF-α对PAI-2表达的调节

Regulatory effect of TNF-α on the expression of PAI-2 in epidermal keratinocytes

目的 探讨表皮角质形成细胞中TNF α对PAI 2表达的调节及其生物学意义。方法 利用免疫细胞化学、TUNEL反应及免疫细胞化学 TUNEL双标等方法 ,观察TNF α作用下 ,培养的表皮角质形成细胞及其脱落细胞中PAI 2表达的变化及凋亡发生的情况。结果 在TNF α作用下 ,脱落细胞中PAI 2的表达明显增加 ,凋亡细胞的数量增多 ,PAI 2在凋亡细胞中的表达也增强 ,而在一些胞体较大、形态呈多角形的凋亡细胞中PAI 2表达的增加更为明显。结论 在高度分化的表皮角质形成细胞中 ,TNF α可促进PAI 2的表达并可诱导凋亡的产生 ,就与KC凋亡的关系而言 ,TNF α所诱导的PAI 2可分为两种 ,一种与KC凋亡有关 ,另一种与KC凋亡无关。

Objective To investigate the regulatory effect of TNF α on the expression of plasminogen activator inhibitor 2(PAI 2) and its biological significance. Methods Using immunocytochemistry(ICC), terminal deoxynucleotidyl transferase mediated end labeling (TUNEL) and ICC/TUNEL double label methods, the expression of PAI 2 in and the apoptosis of the cultured epidermal keratinocytes treated by TNF α were detected. Results After the mouse epidermal keratinocytes were incubated with TNF α, compared with the unshed cells, the expression of PAI 2 in shed cells and the cellular apoptosis increased obviously. The increased PAI 2 expression also appeared in the apoptotic cells, especially in larger and multi angled apoptotic cells. Conclusion In highly differentiated epidermal keratinocytes, TNF α can enhance the expression of PAI 2 as well as apoptosis. Furthermore, according to the relation with the keratinocytes apoptosis, there are two types of PAI 2 induced by TNF α, one of which is related to apoptosis, but the other is not.