17β雌二醇增强小鼠肌原细胞的胰岛素效应

Insulin action potentiation by 17β-estradiol in cultured C2C12 mytoblasts

目的 :探讨 17β雌二醇对小鼠肌原细胞的胰岛素效应的影响和对高胰岛素诱导的胰岛素抵抗的防止作用。方法 :将小鼠肌原细胞分为 6组 ,3组不加高胰岛素 (5× 10 -7mol/L)预处理 2 4h后 ,以其中 1组为对照 ,另 2组分别用 1nmol/L、10nmol/L的 17β雌二醇再处理 2 4h。另外 3组加高胰岛素 (5× 10 -7mol/L)预处理 2 4h之后 ,弃培养液 ,用冷的PBS洗涤细胞 3次 ,即为胰岛素抵抗细胞 ,以其中 1组为胰岛素抵抗细胞组 ,另 2组分别用 1nmol/L、10nmol/L的 17β雌二醇再处理 2 4h。对上述 6组细胞进行胰岛素刺激的葡萄糖摄取能力、糖原合酶 (GS)、磷酸果糖激酶 (PFK)和丙酮酸激酶 (PK)的活性测定。结果 :17β雌二醇可提高小鼠肌原细胞的葡萄糖转运能力 ;提高总糖原合酶活性和糖原合酶的活性 ;还可增强细胞的磷酸果糖激酶和丙酮酸激酶活性。结论

AIM: To investigate the effect of 17β-estradiol on insulin action in cultured C2C12 mytoblasts. METHODS: C2C12 mytoblasts were cultured in 35 mm wells of six-well culture plate in an atmosphere of 5% CO 2 at 37℃ in DMEM supplemented with 10% FBS and penicillin/streptomycin(1×10 5 U/L) to reach 80% confluence. Insulin-resistance C2C12 mytoblasts were obtained by incubating the cells for 24 hours in the presence of a high concentration (5×10 -7 mol/L) of insulin. After treatmented with 17β-estradiol (1 nmol/L and 10 nmol/L, respectively) for 24 hours, C2C12 mytoblasts were performed to measure insulin-stimulated 2-DG uptake and GS, PFK, PK activities. RESULTS: 17β-estradiol enhanced the capacity of insulin-stimulited 2-DG uptake, increased the GS, PFK and PK activities and prevented insulin-induced resistance in cultured C2C12 mytoblasts. CONCLUSION: 17β-estradiol potentiates insulin action and preventes insulin-induced resistance in cultured C2C12 mytoblasts.