喉乳头状瘤患儿血清人乳头状瘤病毒11b病毒样颗粒抗体检测及意义

The detection and significance of human papilloma virus 11b virus like particles and its serum antibody in juvenile larynx papilloma

目的 制备人乳头状瘤病毒 (humanpapillomavirus,HPV) 11b型病毒样颗粒 (viruslikeparticles ,VLP)检测儿童喉乳头状瘤 (juvenilelarynxpapilloma ,JLP)患者血清抗HPV11bVLP抗体 ,探讨与疾病发生、发展的关系。方法 采用重组杆状病毒昆虫细胞系统制备HPV11bVLP。通过酶联免疫吸附试验 (enzymelinkedimmunosorbentassay,ELISA)方法检测 4 6份复发性儿童喉乳头状瘤患儿不同发病时期的血清和 2 0份健康对照组的血清抗体。将标本分 5组 :A组 :健康对照 (2 0份 ) ;B组 :发病 1年(15份 ) ;C组 :发病 2年 (15份 ) ;D组 :未复发 1年 (8份 ) ;E组 :未复发 2年 (8份 )。结果A、B、C、D、E各组血清抗体吸光度 (A)值平均 ( x±s)分别为 0 0 73± 0 0 35 ;0 12 0± 0 0 4 9;0 137± 0 0 5 7;0 5 18±0 12 2 ;0 5 5 7± 0 14 4 ,B、C、D、E各组与A组比较差异均有显著性意义 (P <0 0 5 )。未复发组 (D +F组 )和复发组 (B +C组 )间A均值比较差异有高度显著性意义 (t=14 90 ,P <0 0 0 0 1)。两组年龄比较差异有显著性意义 (6 2 5 0± 1 6 93和 3 2 2 7± 1 4 5 3;t=6 76 7,P <0 0 0 0 1)。结论 HPV感染的JLP患者血清能产生抗HPV抗体 ,疾病发展和预后与血清抗病毒中和抗体水平密切相关。HPV11bVLP及血?

Objective To study the relationship between human papilloma virus 11b virus like particles (HPV11bVLPS) serum antibody and the development and prognosis of juvenile larynx papilloma (JLP). Methods Enzyme linked immunosorbent assay (ELISA) was used to detect the serum HPV11bVLP antibody (Ab) of 46 JLP′s samples in different stage and 20 controls using HPV11bVLPS which was produced by recombinant bacilovirus in insect cells. Grouping: A: control group ( n =20); B: the time of onset was 1 years ( n =15); C: the time of onset was 2 years ( n =15); the patients were followed-up 1 year without recurrence ( n =8); E: The patients were followed-up 2 years without recurrence ( n =8). Results A value of HPV11bVLP Ab among A、B、C、D、E. groups were: (0.073±0.035);(0.120±0.049);(0.137±0.057);(0.518±0.122); (0.557±0.144). There was a significant difference between JLP patients and the control group ( P <0.05). The level of HPV11bVLP Ab in (D+E) group (0.534±0.132) was higher than (B+C) group ( 0.128±0.053) ( t =14.90, P <0.000 1). Conclusion The results suggested that HPV serum antibody was produced in JLP with HPV infection. There is close relationship between the development and prognosis of the disease and the level of HPV11Ab in serum. The assay of serum HPV11bVLPAb and HPV-VLP could be used as immunological study of HPV11-infection associated disease.