升压联合化疗对大鼠脑胶质瘤形态学变化的影响

Effects of chemotherapy with angiotensin II-induced hypertension on transplanted intracerebral rat gliomas

目的 :通过体外细胞化疗和体内对大鼠胶质瘤升压联合化疗后 ,观察体外细胞形态变化和体内肿瘤抑制作用 ,观察变压联合化疗对胶质瘤的治疗效果。方法 :采用胶质瘤体外培养、化疗。体内采用脑定位注射接种法 ,复制大鼠脑胶质瘤动物模型。体内进行联合升压化疗。结果 :体外培养瘤细胞 ,使用化疗药后 ,可见细胞体积增大、胞质呈颗粒样变、胞内容物外溢等形态学变化 ;体内化疗后肿瘤体积变小 ,瘤内可见坏死区等病理变化 ;升压化疗组肿瘤内血流增加、药物浓度增加 ;动物生存期延长。结论 :升压联合化疗对大鼠脑胶质瘤具有明显抑制作用 。

AIM: To observe morphological changes in transplanted intracerebral rat gliomas and rat survival time with gliomas under chemotherapy with angiotensin II-induced hypertension. METHODS: C6 glioma cells were cultured, and the effects of carmustine, nimodipine or/and teniposide on gliomas cells was observed. In addition, the brain tumor model was established in Wistar rats by stereotaxic inoculation of C6 glioma cells. The tumor-bearing rats were treated with carmustine, nimodipine lisplatin or/and teniposide during angiotensin II-induced hypertension, the pathological changes in gliomas was also examined. RESULTS: In vitro experiments showed chemotherapy resulted in morphologic changes in glioma cells, including cell enlargement, degeneration. In vivo experiments, the survival time of tumor-bearing rats was longer, the voume of gliomas was smaller in chemotherapy with hypertension group than those in chemotherapy alone, and pathological examination showed necrosis in the gliomas. CONCLUSION: Chemotherapy with angiotensin II-induced hypertension has a better inhibitory effect on rat intracerebral gliomas than chemotherapy alone.