微血管密度与肝细胞癌侵袭性关系的研究

Study of the relationship between angiogenesis and invasiveness of hepatocellular carcinoma

目的 研究肿瘤微血管密度 (MVD)与肝细胞癌 (下称肝癌 )侵袭性的关系 ,探讨肝癌的生物学特性及侵袭转移机制。方法 将 37例经病理证实的肝癌手术切除标本 ,分为侵袭性肝癌和非侵袭性肝癌 ,用 L SAB免疫组化法利用 F R抗原抗体分别行血管内皮细胞染色计数 MVD。结果 MVD在肝癌组织内呈异质性分布 ,血管形态不规则 ,瘤内 MVD较癌旁区和正常组织区明显增高 (P <0 .0 1,P <0 .0 5 )。两组中侵袭性肝癌组 MVD明显高于非侵袭性肝癌组 (P <0 .0 5 )。 MVD与肝癌 TNM分期有相关性 ,但与肿瘤的大小、有无包膜和分化程度无关。

Objective To study the relationship between angiogenesis and invasiveness of hepatocellular carcinoma(HCC),and to explore the biology and mechanism related to invasiveness and metastasis of HCC Methods Microvesel density(MVD) was detected on archival specimens of 37 HCC by immunohistoc hemical analysis using antibodies against factor Ⅷ Results MVD demonstrated considerable heterogeneity in the HCC tissue MVD in HCC tissue was significantly higher than that in the tumor adjacent and in normal tissue(P<0 01,0 05) 37 cases of HCC were divided into invasiveness and no invasiveness according to satellite nodules and/or portal vein embolus MVD in HCC with invasiveness was higher than that in HCC with no invasivenes(p<0 05) MVD was significantly related to tumor stage statistically(P<0 01) But significant difference in MVD was not found between small and large HCC or between HCC with and without capsule as well as between HCC with good differentiation and with poor differentiation(P>0 05) Conclusion Angiogenesis is related to invasiveness and biology behaviour of HCC