多发性骨髓瘤患者血管内皮生长因子、碱性纤维母细胞生长因子、肝细胞生长因子血清浓度的临床意义

Clinial significance of angiogenic factors VEGF,bFGF,HGF serum concentrations in multiple myeloma

目的 研究血管内皮生长因子 (VEGF)、碱性纤维母细胞生长因子 (bFGF)、肝细胞生长因子 (HGF)与多发性骨髓瘤 (MM )的关系 ,探索其临床意义。方法 用双抗体夹心酶联免疫吸附测定法 ,检测 32例MM患者不同时期的VEGF、bFGF、HGF和白细胞介素 6 (IL 6 )血清浓度 ,观察其与MM分期和治疗效果的关系 ;以正常人为对照组。结果 MM患者治疗前的VEGF、bFGF、HGF血清水平比正常对照组明显增高 ,IL 6血清水平与正常对照组比较差异无统计学意义 ;MMⅠ期、Ⅱ期的VEGF血清水平显著低于Ⅲ期 ,而Ⅰ、Ⅱ期之间的VEGF血清水平对比差异无统计学意义 ;MMⅠ期、Ⅱ期、Ⅲ期的bFGF及HGF血清水平依次增高 ,对比差异有统计学意义 ;各期MM之间的IL 6血清水平对比差异无统计学意义 ;MM治疗有效者 ,其血清VEGF、bFGF、HGF水平比治疗前明显降低 ,而治疗无效者 ,其血清VEGF、bFGF、HGF水平与治疗前比较差异无统计学意义。结论 血管生成调控因子VEGF、bFGF和HGF的血清水平与MM的严重程度及治疗效果相关 。

Objective To study the relationship of angiogenic cytokines vascular endothelial growth factor(VEGF)、basic fibroblast growth factor(bFGF)、hepatocyte growth factor(HGF) with multiple myeloma (MM), and to explore their clinical significances. Methods The serum levels of VEGF,bFGF,HGF and IL-6 were detected by enzyme-linked immunosobant assay technique in 32 patients with MM and in 20 controls (healthy volunteers). The associations between the cytokines serum levels with MM stages (Ⅰ-Ⅲ) and with MM therapeutic effect were observed. Results Pretreatment MM had significantly higher VEGF,bFGF,HGF serum levels than controls. No obvious difference of IL-6 serum level was found between MM patients and healthy volunteers. Pretreatment serum levels of VEGF were significantly lower in stage Ⅰ,Ⅱ than in stage Ⅲ MM, and between stage Ⅰ and stage Ⅱ there was no significant difference. Pretreatment serum levels of bFGF,HGF were significantly elevated in stage Ⅰ,stage Ⅱ and stage Ⅲ, respectively. No significant differences of IL-6 serum level were found between stage Ⅰ,stage Ⅱ and stage Ⅲ MM. In the patients responding to chemotherapy a significant decrease in VEGF,bFGF and HGF serum levels occurred, while in the patients who did not achieve a remission, there was no significant decrease in them. Conclusion The serum levels of angiogenic factors VEGF,bFGF,HGF are correlated with the severity of MM and its therapeutic effect, they could be used for a parameter for determining the severity of MM and its respondence to therapy.