应用基因芯片技术筛查葡萄胎相关基因的研究

Study of DNA microarray chip of associated genes of hydatidiform mole

目的探讨应用基因表达谱芯片技术,筛选葡萄胎发病及恶性转化相关基因的有效性。方法应用含4096条基因的cDNA表达谱基因芯片,对2例完全性葡萄胎组织和与葡萄胎孕周接近的2例正常绒毛组织进行基因表达谱分析,比较两种组织中差异表达的基因。结果2例葡萄胎及2例正常绒毛组织中均有差异表达的基因89条,占所检基因总数的2.2%。与正常绒毛组织比较,葡萄胎组织中基因表达显著增强(上调)的有24条,表达显著降低(下调)的有65条。基因生物学信息分析显示,RASA1、转化生长因子βⅡ型受体、BTG2等与细胞生长抑制有关的基因,在葡萄胎组织中显著下调;而与组织细胞增生和恶性转化及肿瘤转移有关的基因,如胸腺嘧啶核苷激酶1、核苷酸还原酶小亚单位、葡萄糖转运蛋白3、细胞周期蛋白B等基因在葡萄胎组织中显著上调。

Objective To screen genes which may associate with the development and malignant transformation of hydatidiform mole Method The differentially expressed genes were analyzed between the tissues of two cases of hydatidiform mole and the normal placental tissues of two cases of pregnancy which had almost the same pregnant ages as hydatidiform mole, using cDNA chips containing 4 096 genes Results There were total 89 genes significantly differently expressed in all hydatidiform moles, counting for 2.2% of total genes. Compared with normal villi, 24 genes in hydatidiform mole were up regulated and 65 genes were down regulated. Bioinformatical analysis of genes showed genes associated with cell proliferative inhibition, such as Ras GTPase activating protein, TGF betaIIR alpha, BTG2 were significantly down regulated, whereas genes associated with cell proliferation, malignant transformation and tumor metastasis as thymidine kinase, ribonucleotide reductase, glucose transport protein were highly up regulated Conclusion cDNA chip technique is one kind of very effective methods in screening associated genes in hydatidiform mole.