c-erbB-2与c-raf-1反义寡核苷酸联合转染对裸鼠人卵巢癌皮下移植瘤形成的抑制作用

Inhibition effects of c-erbB-2 and c-raf-1 antisense oligodeoxynucleotides combined transfection on the human ovarian carcinoma transplanted subcutaneously in nude mice

目的 探讨c erbB 2与c raf 1反义寡核苷酸 (antisenseoligodeoxynucleotides ,ASODN)联合转染 ,对人卵巢癌细胞株SKOV3 ,在裸鼠皮下移植瘤形成过程中的抑制作用。方法 共分 7组 :Ⅰ组(正常对照 )、Ⅱ组 [c erbB 2正义寡核苷酸 (SODN) ]、Ⅲ组 (c raf 1SODN)、Ⅳ组 (c erbB 2ASODN)、Ⅴ组(c raf 1ASODN)、Ⅵ组 (全剂量联合 )、Ⅶ组 (半剂量联合 )。根据不同分组条件将相应寡核苷酸导入SKOV3细胞株内 ,然后接种于裸鼠皮下 ,观察肿瘤出现的时间和肿瘤体积的变化 ,并计算抑瘤率。结果 Ⅱ、Ⅲ组与对照组成瘤能力比较 ,差异无显著性 ,Ⅳ、Ⅴ组的成瘤能力明显降低 ,Ⅵ组与Ⅶ组的成瘤能力最低 ,Ⅵ组与Ⅶ第 1次出现肉眼可见肿瘤的时间分别为 1 3 7d和 1 5 2d ,最大抑瘤率分别为61 1 %和 71 3 %。结论 反义寡核苷酸联合转染 。

Objective To investigate the inhibition effects of c erbB 2 and c raf 1 antisense oligodeoxynucleotides (ASODN) combined transfection on the human ovarian epithelial cancer transplanted subcutaneously in nude mice Methods There were 7 groups: normal control group,c erbB 2 sense observed group,c raf 1 sense observed group,c erbB 2 antisense observed group,c raf 1 antisense observed group,whole dose combined group,half dose combined group Human ovarian epithelial cancer cells SKOV3 were treated by different oligodeoxynucleotides,then transplanted subcutaneously in nude mice,respectively The changes of tumor volume were observed and the tumor growth inhibitory rate was calculated Results There was no difference between sense observed group and normal control group There was a larger growth inhibitory rate in whole dose combined group and half dose combined group,the first time that can be detected was 13 7 days and 15 2 days,and the maximum tumor growth inhibitory rates were 61 1% and 71 3%,respectively Conclusions The results suggested that ASODN combined transfection can inhibit the tumorigenesis of ovarian epithelial cancer cells in nude mice,it may be a more useful gene therapy for the ovarian epithelial carcinoma