贫铀对小鼠睾丸生精细胞和精子遗传损伤的初步研究

The genetic damage to testicular germ cells and sperms of mice after exposure to Depleted Uranium

目的 初步探讨小鼠吸入不同剂量的贫铀 (DU)气溶胶对生精细胞和精子的遗传损伤作用。方法 采用小鼠睾丸初级精母细胞染色体畸变试验、小鼠睾丸生殖细胞微核试验和精子染色质结构试验 (SCSA) ,评价DU诱导小鼠生精细胞和精子的遗传损伤程度。结果 吸入DU气溶胶组睾丸生殖细胞微核发生率与对照组相比无显著差异 ,吸入 ( 73 1 5 2± 3 5 99)和 ( 877 83± 43 19)ng gDU组小鼠睾丸初级精母细胞染色体数目畸变率为 17 2 %和 2 7 2 %,与对照组比较显著升高 (P <0 0 1) ;精子染色质结构试验中 ,对照组、吸入 ( 73 1 5 2± 3 5 99)和 ( 877 83± 43 19)ng gDU组的精子SCSA各变量平均值 :αT分别为 2 5 3 5 1± 3 1 76、2 88 2 9± 68 3 0、2 94 5 6± 72 90 ,%COMP分别为 3 73± 5 5 0、9 0 2± 4 43、9 11± 5 3 3 ,%GREEN分别为 0 89± 0 44、1 62± 1 0 6、1 80± 0 61,DU组各变量值与对照组比较均有显著增高 (P <0 0 5 )。

Objective To investigate the genetic damage to testicular germ cells and sperms of mice induced by inhalation of Depleted Uranium(DU) aerosol. Methods Genetic damage due to DU to spermatogenic cells and sperms of mice was assessed by chromosome aberration test, micronucleus test and sperm chromatin structure assay(SCSA) of the primary spermatocytes of mice. Results No difference in micronucleus rate of testicular germ cells was found between DU aerosol group and the control group The chromosome numerical aberration rates of the groups inhaling DU aerosol at the doses of (731 52±35 99) ng/g and (877 83±43 19) ng/g were 17 2% and 27 2%, respectively, increasing significantly as compared with that of the control group( P <0 01) SCSA revealed that the average α T values, %COMP values and %GREEN values in DU aerosol groups increased significantly than those in the control groups( P <0 05) Conclusion Inhalation of DU aerosol may result in genetic damage to testicular germ cells and sperms of mice