PKC在LPS激活大鼠肺巨噬细胞核转录因子-κB中的作用

Roles of PKC in LPS-induced NF-κB activation of macrophages in rats

目的 探讨蛋白激酶C(PKC)在LPS激活肺巨噬细胞核转录因子 κB信号传导通路中的作用。方法 采用夹心ELISA的方法检测巨噬细胞NF κB的核内浓度 ;免疫组化染色观察LPS作用前后NF κB的位置改变。结果 LPS刺激可诱导巨噬细胞NF κB活化 ,二者之间存在时效与量效的依赖关系 ;免疫组化染色显示LPS作用后 ,NF κB发生核内移位 ,由胞浆进入细胞核内 ;而特异性PKC抑制剂 (CalC)和NF κB阻断剂 (PDTC)均能在不同程度上抑制LPS的作用。结论 PKC作为NF κB活化的上游信使 ,参与了LPS激活NF

Objective To study the roles of protein kinase C (PKC) in the signal transduction pathway of LPS induced nuclear transcription factor (NF) κB activation of alveolar macrophages(AMs). Methods NF κB level in nuclear protein extraction of AMs was detected by sandwich ELISA. Intranuclear translocation of NF κB was observed by immunocytochemical staining. Results LPS could induce NF κB activation of AMs in time and dose dependent manners. Immunohistochemical staining revealed intranuclear translocation following LPS induction. Specific calphostin C (Cal C) and pyrrolidine dithiocarbamate (PDTC) could inhibit LPS induced NF κB activation. Conclusion PKC, as a up stream messenger, is involved in the signal transduction pathway of LPS induced NF κB activation.