摘要
目的:测定复方黄连素片中盐酸小檗碱的含量。方法;采用高效液相色谱法。色谱柱为Nova-pak C_(18)柱(5μm,250mm×4.6mm);流动相为:乙腈-磷酸盐缓冲液(O.05mol/L磷酸二氢钾和0.05mol/L庚烷磺酸钠(1:1),(40:60),含0.2％三乙胺),用磷酸调节pH值至3.0;流速:1.0ml/min;检测波长263nm;柱温:室温。结果:盐酸小檗碱在0.48μg～1.12μg范围内具有良好线性关系,平均回收率为100.4％,RSD=1.8％。结论:该法可用于含有盐酸小檗碱复方制剂的含量测定方法。
To improve the method of berberine deternination in compound berberine tablets. Methods: A HPLC method was established by using Nova-pak C18 colum (250mm×4. 6mm, 5μm)as analytical column, the mobile phase was MeCN-phosphoric buffer(0. 05mol·L-1 KH2PO4 and 0. 05mol· L-1 Sodium Heptanesulfonate(1:1), (40 : 60) .contain 0. 2% triethylamine), adjust PH to 3.0 by phosphoric acid. The flow rate was 1.0ml · min-1,and detection wavelength was at 263nm. ResultS:The linear range of berberine was 0. 48-1. 12μg,and the recovery of the method was 100. 4% with RSD = 1. 8%. Conclution:The method is accurate and suitable for the analysis of berberine in compound preparation.
出处
《中国药品标准》
CAS
2004年第1期22-23,共2页
Drug Standards of China
