期刊文献+

Amperometric Biosensor for Hydrogen Peroxide Based on Electrodeposited Sub-micrometer Gold Modified Glassy Carbon Electrode

Amperometric Biosensor for Hydrogen Peroxide Based on Electrodeposited Sub-micrometer Gold Modified Glassy Carbon Electrode
原文传递
导出
摘要 A new type of hydrogen peroxide amperometric biosensor was fabricated based on electrochemically deposited sub-micrometer Au particles (sm-Au) on a glassy carbon electrode (GCE). Electrochemical deposition condition was optimized for obtaining uniformly distributed sub-micrometer sized Au array on the electrode surface. The hy-drogen peroxide sensor was fabricated by adsorbing phenothiazine methylene blue (MB) molecules on the surface of sm-Au and covering a cross-linked horseradish peroxidase (HRP) layer, labeled as HRP/MB/sm-Au/GCE. The characteristics of this biosensor were evaluated with respect to applied potential and pH. The amperometric re-sponse of the sensor was linear to the H2O2 concentration over a wide range of 9.9×10-61.11×10-2 mol/L. A detection limit (s/n=3) of 3.0×10-6 mol/L H2O2 was estimated for a sampled chronoamperometric detection at 1.5 min after potential step of 200 to -400 mV vs. SCE. The immobilized MB molecules shuttled electrons at a=0.77 and an apparent electron transfer rate constant of 0'sk=0.053 s-1. Interference of ascorbic acid, dopamine and uric acid was investigated. This sensor has very good stability and reproducibility for long-term use. A new type of hydrogen peroxide amperometric biosensor was fabricated based on electrochemically deposited sub-micrometer Au particles (sm-Au) on a glassy carbon electrode (GCE). Electrochemical deposition condition was optimized for obtaining uniformly distributed sub-micrometer sized Au array on the electrode surface. The hy-drogen peroxide sensor was fabricated by adsorbing phenothiazine methylene blue (MB) molecules on the surface of sm-Au and covering a cross-linked horseradish peroxidase (HRP) layer, labeled as HRP/MB/sm-Au/GCE. The characteristics of this biosensor were evaluated with respect to applied potential and pH. The amperometric re-sponse of the sensor was linear to the H2O2 concentration over a wide range of 9.9×10-61.11×10-2 mol/L. A detection limit (s/n=3) of 3.0×10-6 mol/L H2O2 was estimated for a sampled chronoamperometric detection at 1.5 min after potential step of 200 to -400 mV vs. SCE. The immobilized MB molecules shuttled electrons at a=0.77 and an apparent electron transfer rate constant of 0'sk=0.053 s-1. Interference of ascorbic acid, dopamine and uric acid was investigated. This sensor has very good stability and reproducibility for long-term use.
出处 《Chinese Journal of Chemistry》 SCIE CAS CSCD 2004年第4期360-364,共5页 中国化学(英文版)
基金 theNationalNaturalScienceFoundationofChina(No.20075025).
关键词 hydrogen peroxide amperometric biosensor sub-micrometer gold glassy carbon electrode me-thylene blue horseradish peroxidase hydrogen peroxide, amperometric biosensor, sub-micrometer gold, glassy carbon electrode, me-thylene blue, horseradish peroxidase
  • 相关文献

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部