乳腺不典型增生及乳腺癌中层粘连蛋白及纤维连接蛋白的表达及意义

Laminin and fibronectin expressions in atypical hyperplasia and breast cancer and the significance

目的 观察层粘连蛋白 (Laminin ,LN)和纤维连接蛋白 (Fibronectin ,FN)在乳腺不典型增生及乳腺癌中表达的变化。方法 用S P免疫组化染色法分别检测正常乳腺组织、Ⅰ、Ⅱ、Ⅲ级不典型增生的乳腺组织及乳腺癌组织中LN和FN的表达情况。结果 正常乳腺组织中LN只在基底膜表达 ;FN表达于基底膜及少量间质细胞。Ⅰ级增生的乳腺组织中LN主要表达于基底膜及上皮细胞 ;FN表达近似正常组织。Ⅱ级不典型增生中LN于上皮细胞表达增多 ;FN主要表达于基底膜及上皮细胞。Ⅲ级不典型增生中LN的间质表达明显增多 (P <0 0 5 ) ,而上皮细胞表达明显减少 (P <0 0 5 ) ;FN则主要表达于上皮细胞及增生腺管周围的基质中。浸润性导管癌中LN主要表达于癌细胞 ,间质表达显著减少 (P <0 0 5 ) ;FN亦主要表达于癌细胞中。结论 乳腺不典型增生时LN表达部位的变化反映了乳腺上皮细胞的增生状态 。

Objective To investigate the changes of laminin(LN) and fibronectin(FN) expressions in atypical hyperplasia and breast cancer. Methods LN and FN expressions in normal breast tissue, tissues from grade Ⅰ, Ⅱ and Ⅲ atypical hyperplasia and breast cancer were detected by means of S P immunohistochemical staining method. Results In normal breast tissue, LN expression was found only in basement membrane, but FN expression in basement membrane and matrix. In gradeⅠ hyperplasia, LN expression was found in basement membrane and epithelium, but FN expression was similar to that in normal tissue. In grade Ⅱhyperplasia, more LN expression was found in epithelium, but FN expression in epithelium and matrix. In grade Ⅲ hyperplasia, LN expression increased significantly in matrix and decreased significantly in epithelium as compared with those in grade Ⅱ( P <0 05), but FN expression was found in epithelium and matrix around the hyperplastic duct. In infiltrating duct carcinoma, LN expression was found mainly in cancer cells, but less expression in matrix ( P <0 05), but FN expression was found mainly in cancer cells, too. Conclusion Changes of position dependent laminin expression may reflect the status of hyperplasia in breast epithelium. Expression of fibronectin in matrix in hyperplasia is a defensive reaction.