肿瘤细胞中ASPP_2 mRNA的表达及意义

ASSP_2 mRNA expression in tumor cells and its significance

目的 建立p5 3凋亡刺激蛋白基因 (ASPP2 )mRNA表达水平的RT PCR测定方法 ;并应用该方法测定ASPP2 mRNA在正常人血液单核细胞与人淋巴瘤细胞株jurkat及成纤维细胞和结肠癌细胞株HT 2 9的表达水平。方法 应用单核细胞分离液分离正常人血液单核细胞 ,用DMEM培养液培养细胞株jurkat、成纤维细胞和结肠癌细胞株HT 2 9,所得细胞用Tripure分离试剂提取总RNA。最后用RT PCR检测ASPP2 mRNA的表达水平。结果 ASPP2 mRNA在正常人血液单核细胞中的表达水平比人淋巴瘤细胞株jurkat的表达水平高 ,而正常人成纤维细胞中ASPP2 的表达水平比结肠癌细胞株HT 2 9低。结论 ASPP2 的mRAN表达量是在p5 3野生型的肿瘤细胞中降低而不是在p5 3突变型的肿瘤细胞中 ,说明ASPP2 调节着p5

Objective To establish a reverse transcription PCR (RT PCR) assay to measure the expression levels of apoptosis stimulating protein of p53(ASPP2) mRNA in normal blood monocytes, Jurkat cell line, fibroblasts, colon cancer HT 29 cell line. Methods Monocytes in normal blood were isolated using monocyte isolation reagent. Jurkat cells, fibroblasts and HT 29 cell line were cultured using DMEM culture medium. Total RNA was extracted from the harvested cells using Tripure TM isolation reagent. ASPP2 mRNA expression level was determined by RT PCR. Results ASPP2 mRNA expression level was higher in normal blood monocytes than that in Jurkat cell line but lower in human fibroblasts than that in HT 29 cell line. Conclusion The mRNA expression of ASPP2 is down regulated in human tumor cell expressing wild type p53 but not mutant p53, suggesting that ASPP2 can regulate the tumor suppression of p53.