冠心病患者血浆同型半胱氨酸与单核细胞趋化蛋白-1水平的变化

Changes of plasma levels of homocysteine and MCP-1 in patients with coronary artery disease

目的 观察冠心病患者血浆同型半胱氨酸和MCP 1浓度的变化 ,并分析Hcy对血浆MCP 1表达的影响。方法 所有入选的研究对象均经冠状动脉造影检查 ,其中冠心病组 70例 ,对照组 5 0例。应用高压液相色谱法测定血浆Hcy浓度 ,标准方法测定血浆丙二醛 (MDA)浓度 ,ELISA法检测血浆MCP 1浓度。结果 ①冠心病组血浆Hcy、MDA及MCP 1浓度均显著高于对照组 (P <0 .0 5 )。②冠心病组患者的血浆同型半胱氨酸浓度与MCP 1浓度呈明显线性相关。结论 高同型半胱氨酸血症可能通过提高MCP 1的表达、增加脂质过氧化损伤 。

Objective To investigate the changes of plasma levels of homocysteine (Hcy) and monocyte chemoattract protein 1 (MCP 1) in coronary artery disease (CAD) patients and to analyze the effect of Hcy on the expression of MCP 1. Methods The CAD diagnosis of all the studied subjects was confirmed by coronary angiography. A total of 70 patients with CAD and 50 control subjects were recruited in the study. Plasma Hcy concentration, plasma malondialdehyde (MDA) and plasma MCP 1 were determined by high performance liquid chromatography (HPLC), standard protocols and ELISA, respectively. Results Plasma levels of Hcy, MDA and MCP 1 in patients with CAD were significantly higher than those in the control subjects ( P <0 05). A linear correlation between Hcy and MCP 1 levels of the CAD patients was observed. Conclusion Hyperhomocysteinemia might promote the development of atherosclerosis through the induction of MCP 1 production and recruitment of monocytes into the subendothelium.