同型半胱氨酸、同型半胱氨酸诱导基因抗体及叶酸对人胚心肌细胞同型半胱氨酸诱导基因的表达及细胞增殖的影响

Effects of homocysteine,homocysteine induced gene antibody and folic acid on HCY-2 expression and proliferation of human embryo cardiac myocytes

目的 :研究同型半胱氨酸 (HCY)、同型半胱氨酸诱导基因抗体 (HCY -2Ab)及叶酸对人胚心肌细胞HCY 2表达及对心肌细胞增殖的影响。方法 :采用免疫细胞化学、RT PCR法体外测定人胚心肌细胞内HCY 2表达 ;用细胞计数法和MTT法对心肌细胞增殖的变化定量分析。结果 :当HCY的浓度小于 1 .2 5mmol/L时 ,HCY 2的表达随着HCY浓度的增高而逐渐增强 ,HCY促进细胞的增殖 ;当HCY浓度超过 1 .2 5mmol/L时 ,则HCY 2的表达逐渐降低 ,HCY抑制细胞的增殖 ;加入HCY 2Ab或叶酸 ,HCY 2的表达减弱 ,同时抑制细胞的增殖。结论 :HCY 2高表达时HCY对心肌细胞的增殖起促进作用 ;HCY 2低表达时则HCY、HCY 2Ab或叶酸抑制心肌细胞的增殖。

Objective:To study the effects of homocysteine(HCY), homocysteine induced gene antibody(HCY-2Ab) and folic acid on HCY-2 expression and proliferation of human embryonic cardiac myocytes. Methods:Immunocytochemistry and RT-PCR were used to analyze the HCY-2 expression on cultured human embryo cardiac myocytes; the cell counting and MTT were used to analyze the proliferation of human embryo cardiac myocytes quantitatively. Results:When the concentration of HCY was less than 1.25 mmol/L, the expression of HCY-2 and the cell proliferation increased as the concentration of HCY increased. The cell proliferation and the expression of HCY-2 were inhibited when the HCY exceeded 1.25 mmol/L. When adding HCY-2Ab or folic acid, the expression of HCY-2 decreased and the cell proliferation were inhibited. Conclusion:Myocyte proliferation is stimulated by HCY when HCY-2 is highly expressed,and the proliferation is inhibited by HCY, HCY-2Ab and folic acid when HCY-2 is weakly expressed.