霍乱毒素及外周神经对视神经远端切断后视网膜谷氨酸能节细胞再生的作用

Effects of colera toxin and peripheral nerve on regeneration of glutamate immunoreactive retinal ganglion cells after distal axotomy of optic nerve

目的 :探讨霍乱毒素 (CTx)及外周神经对成年金黄地鼠远端视神经受损后视网膜谷氨酸 (Glu)能节细胞(RGCs)再生的作用。方法 :远端切断视神经并缝接自体坐骨神经 (AG) ,玻璃体内注射CTx及 /或植入小段坐骨神经分支 (SN)。动物分为AG +CTx组 ;AG +SN组 ;AG +SN +CTx组 ,分别存活 4W、5W ,荧光金和免疫荧光组织化学双标法标记再生的RGCs。结果 :术后 5W ,AG +CTx组 ;AG +SN组 ;AG +SN +CTx组Glu免疫反应阳性RGCs再生数分别占再生总数的 4.2 5 %、2 .5 0 %及 6.0 0 % ,AG +SN组与AG +SN +CTx组间差异显著。结论 :CTx与SN能协同促进视神经远端切断后Glu能节细胞的再生 .

Objective:To investigate the effects of colera toxin(CTx) on the regeneration of glutamate(Glu) immunoreactive retinal ganglion cells (RGCs) after distal axotomy in adult hamsters. Methods:After optic nerve (ON) transected intracranically, an autologus sciatic nerve was removed and connected to the proximal stump of the ON(attached graft, AG). CTx was injected and/or a 2 mm segment of sciatic nerve was inserted intravitreally. Animals were divideded into 3 groups: AG+SN group,AG+CTx group,AG+SN+CTx group. Animals survived for 4-5 weeks respectively. The Flou-Gold(FG) retrograde labeling method and FITC-immunofluorescence labeling method were used to estimate the effects of CTx and SN on promoting the regenerating of Glutamate immunoreactive RGCs in different groups. Results:The Glutamate immunoreactive regenerating RGCs appeared 5 weeks later after operation. The mean number of regenerating Glutamate immunoreactive RGCs in AG+CTx group, AG+SN groups and AG+SN+CTx group were 4.25%, 2.50% and 6.00% respectively. Conclusion:CTx and SN can promote the regeneration of Glutamate immunoreactive RGCs after distal axotomy.