菘蓝对小鼠胚胎发育的保护作用

Protective effects of chloroform extracts from Isatis indigotica leaves on mouse embryo development

目的 :研究菘蓝叶氯仿提取物对环磷酰胺 (CP)引起的胚胎发育障碍的保护作用。方法 :采用小鼠胚胎致畸试验 ,观察了受孕母鼠的体重变化 ,活胎率 ,死胎率 ,吸收胎率 ,以及胎鼠的身长、尾长、体重等指标。并用骨骼染色法观察胎鼠有无骨骼畸形。结果 :菘蓝叶氯仿提取物本身对胚胎发育无毒性 ,而对CP引起的胚胎发育障碍有一定的保护性抑制作用。CP +菘蓝叶提取物 1 0 0mg·kg- 1·w- 1剂量组与阳性对照组相比可显著增加胎鼠的活胎率 (增加 5 9.60 % )、降低吸收胎率 (降低 86.91 % )、及胎鼠畸形率的发生 ,也可明显增加胎鼠的身长、尾长和体重等指标。结论 :中药菘蓝具有保护小鼠胚胎发育的作用。

Objective:To study the protective effects of chloroform extracts from Isatis indigotica leaves on the damage of embryo development induced by cyclophosphamide (CP) in mice. Methods:The parent mice weight, fetus weight and the fetus body and tail length were measured; the survival, death and absorb rates of fetus were calculated using embryonic teratogenicity tests. The fetus skeletal development was observed by skeletal staining. Results:Isatis indigotica had no embryotoxicity, but had significantly protective effects on embryonic development induced by CP. The group of chloroform extracts at the dose of 100 mg/kg·w+CP 10 mg·kg -1 ·w -1 could obviously increase the survival rate (59.60%), decrease absorb (86.91%), death and malformed rate. Furthermore, it could also increase the fetus body and tail length as well as fetus weight. Conclusion:The Chinese medicine Isatis indigotica has the protective effects on embryonic development induced by CP in mice.