慢性乙型肝炎病毒基因型与BCP区变异的临床研究

Clinical study on the relationships between HBV genotype and HBV core promoter mutants

为了研究乙型肝炎病毒 (HBV)基因型与C基因启动子 (BCP)基因变异的关系 ,对 6 9例慢性乙型肝炎患者分别用聚合酶链反应 (PCR)—限制性片段长度多态性 (RFLP)技术和PCR微板核酸分子杂交技术 ,进行基因分型及HBVBCP基因变异检测。在 6 9例慢性乙型肝炎患者中 ,各基因型发生的BCP区A176 2T/G176 4A双突变分别为C型 18例 (4 3 9% )B型 3例 (16 7% )D型 2例 (2 0 % )。C型的BCP双突变率明显高于B型 ,两者相比有显著性差异(P <0 0 5 )。故可以认为乙型肝炎病毒基因型与BCP区双突变存在有一定的相关性。推测A176 2T/G176

In order to investigate the relationships between hepatitis B virus (HBV) genotype and HBV core promoter mutants. Serum samples from 69 infected patients were determined for HBV genotype by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) methods and for HBV basic core promoter(BCP) gene mutants by PCR-microplate nucleotide acid hybridization-ELISA methods. By PCR-RFLP, HBV from 69 patients with hepatitis B can be divided into three type, i.e. type B, C and D. Viral genotype C was identified in 41 cases, genotype B in 18 and genotype D in 10 case, the percentages of type B type C and type D were 69.4%, 26.1%, 14.4% respectively. Twenty-three of 69 HBV infected patients were found to have mutants in HBV BCP region at nucleotide 1762/1764, among which 18 cases were detected with genotype C, 3 cases with genotype B, and in 2 cases with genotype D. The mutation in BCP region at nucleotide 1762/1764 was significantly more frequent in genotype C than B patients (43.9% vs. 16.7%,P<0.05),but HBV BCP gene mutants was not found to have significant different between genotype D and genotype C or genotype B(P>0.05). The results show genotype of Lanzhou mainly consists of type C and type B, genotype D are rare. There were a clear link between HBV genotype and HBV BCP gene mutations at nucleotide 1762/1764, HBV BCP mutations may be a new reason that genotype C have severe hepatitis HBV BCP mutations may be a new reason that genotype C have severe hepatitis disease.