商陆抗病毒蛋白体外抑制HBV的实验研究

The effects of pokeweed antiviral protein from seeds on human hepatitis B virus invitro

探讨商陆抗病毒蛋白 (pokeweedativiralproteinPAP)体外抗乙型肝炎病毒 (HBV)的作用。不同浓度的PAP-S(pokeweedantiviralproteinfromseeds)作用于HepG2 2 15 ,应用ELISA、荧光定量PCR ,分别检测药物作用后细胞上清液的HBsAg、HBeAg、HBVDNA水平的变化。PAP -S处理HepG2 2 15后 72h发现 ,PAP -S在 >1μg/ml时有明显的抗HBV效应 ,在所试验浓度范围 (1- 10 ) μg/ml内 ,倒置显微镜下未发现明显的细胞毒性 ,PAP -S为 5 0 μg/ml时 ,发现部分细胞脱壁。PAP -S对HBsAg、HBeAg、HBVDNA呈剂量时间依赖性。PAP

Effects of pokeweed antiviral protein from seeds (PAP-S) on hepatitis B virus in vitro were evaluated by HepG2.2.15 cell line transfereted with hepatitis B virus DNA. Different concentrations of PAP-S were added into cultured medium respectively. ELISA and Fluorescent quantified PCR were used for determining HBsAg, HBeAg and HBV DNA in culture medium respectively at 48h and 72h. The results showed that the inhibition of PAP-S for HBsAg, HBeAg and HBV DNA was dose-dependent and time-depended. The repressive effect became more potent with increasing PAP concentration. At 72h after PAP-S does with HepG2.2.15 cell, we discover that when the concentration of PAP-S>1μg/ml it has obvious anti-HBV effect. With increasing PAP concentration cytotoxicity of its appeared. No obvious cytotoxicity was discovered under the inverted microscope when concentration of PAP-S from 0.1μg/ml to 10μg/ml, but at 50μg/ml, a part of cells fall from the wall. So it's probably that PAP-S at concentration from 1μg/ml to 50μg/ml much more adapt to anti-HBV than PAP-S>50μg/ml. PAP-S has anti-HBV potential activity inhibitory in vitro.