血管内皮生长因子、胰岛素样生长因子-Ⅱ对肝细胞癌的诊断价值

Clinical evaluation of serum vascular endothelial growth factor and Insulin-like growth factor-Ⅱ assay in the diagnosis of hepatocellular carcinoma

了解血管内皮生长因子 (VEGF)、胰岛素样生长因子 -Ⅱ (IGF -Ⅱ )在肝细胞癌 (HCC)中的诊断意义。分别用双抗体夹心法 (ELISA)及放射免疫测定法 (RIA)测定了 35例肝细胞癌 (HCC)、16例肝硬化 (LC)和 16例慢性肝炎 (CH)及 10例正常对照者 (NC)的血清VEGF、IGF -Ⅱ水平。HCC患者血清VEGF、IGF -Ⅱ的水平明显高于正常对照组及其它各病组 (P <0 0 1或P <0 0 5 ) ,二者对于AFP阴性的HCC诊断阳性率分别为 6 3 6 %和 5 4 5 % ,三者联合检测可提高HCC诊断阳性率达 94 3%。有远处转移的HCC患者VEGF水平明显高于无转移者 (P <0 0 1)

To study the clinical significiance of serum vascular endothelial growth factor (VEGF) and insulin-like growth factor-Ⅱ (IGF-Ⅱ) assay in the diagnosis of hepatocellular carcinoma(HCC). Serum VEGF and IGF-Ⅱ concentration was determined in 35 patients with HCC, 16 patients with (LC), 16 patients with (CH) and 10 normal controls by means of enzyme linked immunosorbent assay system(ELISA) and radioimmunoassay (RIA) respectively. The level of serum VEGF and IGF-Ⅱ of HCC patients were significiance higher than those in normal controls, LC patients and CH patients (P<0.01 or P<0.05),Serum VEGF level was elevated in 22 of 35 (62.8%) HCC patients. The diagnostic positivity was 63.6% in AFP negative HCC patients. Serum IGF-Ⅱ level was elevated in 21 of 35 (60%) HCC patients. The diagnostic positivity was 54.5% in AFP negative HCC patients. The total positive detection rate of HCC with combined VEGF, IGF-Ⅱ and AFP reached 94.3%. The level of serum VEGF in HCC patients with metastasis was significiantly higher than those without metastasis (P<0.01). Above results suggest that serum VEGF and IGF-Ⅱ might be a candidate for a tumor marker for diagnosis of HCC, especially HCC patients with AFP negative. It is helpful in diagnosing HCC earlier and can differentiate HCC from liver cirrhosis. Serum VEGF level of HCC appears to reflect its potential activity of vascular invasion and metastasis.