参芪心复康对心气虚细胞模型β1受体基因表达的影响

Effects of Shenqixinfukang on Expression ofβ_1-Receptor Gene in Cell Model of Heart Qi Deficiency Syndrome

目的 :研究参芪心复康对心气虚细胞模型 β1受体基因表达的影响。方法 :采取“培养心肌细胞缺氧再给氧损伤”的方法建立心气虚细胞模型。设空白对照组、模型对照组、人参组、水蛭组、丹参组、参芪心复康组。采用逆转录聚合酶链反应 (RT PCR)基因表达检测心气虚细胞模型和各中药治疗组的 β1受体基因表达情况。结果 :各中药组 β1受体mR NA表达与心气虚的细胞模型的 β1受体mRNA表达比较差异有显著性 (P <0 0 1 ) ,参芪心复康组与其他中药组的 β1受体mRNA表达情况比较差异有显著性 (P <0 0 1 )。结论 :参芪心复康防治心气虚证的机制与其干预 β1受体mRNA表达有关。

Objective: To study the effects of Shenqixinfukang on expression of β 1 receptor gene in cell model of heart qi deficiency syndrome. Method: The cell model was built by myocardial cell culture method with oxygen deprivation and recovery injury. There were six groups: Blank control group, model control group, Ginseng Group, Leech Group, Danshen Group and Shenqixinfukang group. The RT PCR was used to test the expression of β 1 receptor of all the groups. Result: Compare to the model control group the expression of β 1 receptor of the groups dealt with herbal medicine possessed significant difference ( P <0.01); And among the herbal medicine groups, the expression of β 1 receptor of Shenqixinfukang group also possessed significant difference compare to other groups. Conclusion: The mechanism of Shenqixinfukang in preventing and treating heart qi deficiency syndrome is related to its intervention into the mRNA expression ofβ 1 receptor.