摘要
目的 研究大鼠脑缺血再灌注后蛋白激酶C(proteinkinaseC ,PKC)在海马和顶叶皮质的表达 ,探讨降钙素基因相关肽 (CGRP)和神经生长因子 (NGF)对脑组织缺血再灌注的保护作用及机制。方法 采用颈动脉负压分流法制作大鼠脑缺血再灌注模型 ,采用免疫组织化学SABC法及显微图像分析检测海马及皮质内PKC的表达。结果 大鼠缺血再灌注海马CA1区及顶叶皮质内PKC阳性产物平均灰度值较正常组低 (P <0 .0 5 ) ,注射CGRP或NGF后PKC阳性产物平均灰度值明显高于缺血再灌注组 (P <0 .0 1) ,二者联合应用时平均灰度值比单独应用高 (P <0 .0 1)。结论 CGRP及NGF参与缺血神经元PKC的调节 。
Objective To investigate the expression of protein kinase C (PKC) in hippocampus and parietal cortex after whole cerebral ischemia and reperfusion in rats to explore the protective effects and mechanism of calcitonin gene-related peptide (CGRP) and nerve growth factor (NGF) on ischemic brain tissue. Methods An novel model of whole cerebral ischemia and reperfusion induced by carotid artery negative pressure was extablished in rats, the expression of PKC was detected with SABC immunohistochemical method and analyzed by microimage system. Results The average gray values of PKC positive product in hippocampus CA 1 and parietal cortex were lower in cerebral ischemia/reperfusion group than in sham control group (P<0.05). The average gray values of PKC positive product in CGRP or NGF group were higher compared with cerebral ischemia/reperfusion group (P<0.01). The average gray values of PKC was higher in NGF&CGRP group than in CGRP or NGF group respectively (P<0.01). Conclusion The results indicate that CGRP and NGF participate in the regulation of expression of PKC in ischemic neurons,and they may cooperate with each other.
出处
《解剖科学进展》
CAS
2004年第1期50-53,共4页
Progress of Anatomical Sciences
基金
辽宁省自然科学基金资助项目 ( 6190 19)
