多肿瘤组织芯片在评价肿瘤细胞增殖与凋亡状态研究中的作用

Multiple-tumor tissue microarray and its preliminary application in evaluation of proliferation and apoptosis in cancer cells

目的 :研究多种常见肿瘤组织芯片在观察癌细胞增殖和凋亡中的作用。方法 :在病理档案材料中选取 10种常见恶性肿瘤及其癌旁或正常组织的石蜡包埋标本 ,HE染色切片定位穿刺部位 ,每一蜡块穿取直径 0 6mm大小组织柱 ,排布成 196点列阵的芯片 ;用ki 67、p5 3、rasp2 1、bcl 2和bax单抗SP法进行免疫组化染色 ,对各切片阳性细胞进行计数观察。结果 :组织芯片经染色后结构完整 ,在肿瘤组织中ki 67、p5 3和rasp2 1标记的阳性细胞比癌旁和正常组织明显增高 ;而大多数肿瘤中bcl 2和bax表达呈负相关 ,即bcl 2阴性或低表达者 ,则bax呈不同程度阳性表达 ,反之则相反。结论 :组织芯片技术可以同时用于评价多种不同类型肿瘤样本的增殖活性和凋亡状态 。

OBJECTIVE:To study the methodology of tissue microarray (TMA) technology and its application in evaluation of proliferative activity and invasive behavior of cancer cells. METHODS:Beecher tissue arrayer was used to create a 196 dot tissue microarray, derived from 99 cases of common carcinomas, and their para cancerous tissues and corresponding normal tissues which were formalin fixed and paraffin embedded. Hematoxylin eosin and immunohistochemical stains were performed to evaluate the morphology and expression of ki 67, p53, rasp21, bcl 2 and bax in the specimens. The labeling index (LI) was calculated in the different groups of the tissues.RESULTS: Histological appearance was well preserved in the most dots of the TMA section. LI of ki 67, p53 and rasp21 in low differentiated carcinoma was much higher than those in well differentiated ones and para cancerous and normal tissues. In most of cancers, there was a negative correlation between the expressions of bcl 2 and bax, with a significant difference from those in normal tissues. CONCLUSIONS:Multiple tumor TMA is a simple and easy doing method, which can be widely used in evaluation of proliferation and apoptosis of cancer cells in large scale specimens, and it provides more information to judge the patients’ prognosis.