三氧化二砷诱导人鼻咽癌细胞凋亡的实验研究

Experimental studies of apoptosis induced by arsenic trioxide in human nasopharyngeal carcinoma cells

目的 :研究三氧化二砷 (As2 O3 )体外抑制人鼻咽癌细胞株 (CNE1)增殖及诱导凋亡的作用。方法 :以不同浓度的As2 O3 处理CNE1,用四甲基噻唑蓝 (MTT)法测定细胞增殖情况 ,细胞染色方法观察细胞凋亡的形态 ,原位末端标记法 (TUNEL)检测凋亡细胞 ,流式细胞仪检测细胞的DNA含量。结果 :As2 O3 明显抑制人鼻咽癌细胞株CNE1细胞的增殖 ,其抑瘤作用优于顺铂 (DDP)和 5 -氟尿嘧啶 (5 FU)。As2 O3 作用于细胞后 ,可见典型凋亡细胞的形态学改变 :细胞核固缩 ,染色质凝集 ,核碎裂 ,凋亡小体形成等。流式细胞仪DNA线条图上出现典型的亚二倍体“凋亡峰”。结论 :As2 O3

OBJECTIVE:To investigate the effect of a wide range of concentrations of arsenic trioxide (As 2O 3) on human nasopharyngeal carcinoma cell strain in vitro .METHODS:CNE1 cell strains were dealt with As 2O 3 at different concentrations.Cell proliferation was evaluated by the MTT assay, and the cell apoptosis was evaluated by the cell morphology,flow cytometry and TUNEL methods.RESULTS:The cell proliferation was obviously depressed by As 2O 3 in vitro , which indicated that the depressive effect of As 2O 3 in cancer was better than DDP or 5 FU. The typical morphological features such as nuclear pycnosis, chromatin coagulation, nuclear fragmentation and apoptotic body formation were observed and a typical hypodiploid apoptosis peak in the DNA line was found in the flow cytometry.CONCLUSIONS:As 2O 3 can depress the proliferation of CNE1 and accelerate the apoptosis in vitro .