胃癌组织中环氧化酶-2的表达与微血管密度和VEGF的关系

Relationship of microvessel density,vascular endothelial growth factor and cyclooxygenase-2 expression in human gastric cancer

目的 :探讨环氧化酶 -2 (COX 2 )在胃癌组织中的表达及其与微血管密度(microvesseldensity ,MVD)和血管内皮生长因子 (vascularendothelial growthfactor ,VEGF)的关系。方法 :采用免疫组织化学法 ,检测 43例胃癌患者手术切除标本中COX 2和VEGF的表达 ,并计数MVD。结果 :COX 2在胃癌组织的阳性表达率为60 5 % ,明显高于对照组 ,P <0 0 1;并且与胃癌淋巴结的转移和临床分期有关 ,与肿瘤大小、浸润深度、分化程度均无关。COX 2和VEGF表达一致符合率为 5 3 5 % ,两者表达有显著相关性 ,P <0 0 1。COX 2阳性和VEGF阳性组MVD值 (2 2 0 8± 3 69、2 2 12± 3 5 0 )均高于COX 2阴性和VEGF阴性组 (17 68± 3 5 0、16 15± 3 2 3 ) ,P <0 0 5 ;COX 2和VEGF均为阳性者的MVD值最高 (2 2 86± 3 40 ) ,P <0 .0 5。结论 :COX 2、VEGF的表达以及MVD的测定可作为判断胃癌恶性潜能的重要生物指标。COX 2、VEGF的表达对肿瘤血管形成可能起重要作用 ,联合检测COX 2。

OBJECTIVE:To investigate the expression of cyclooxygenase 2 (COX 2) in gastric cancerand and its relations with the microvessel density (MVD) and vascular endothelial growth factor (VEGF). METHODS:The expressions of COX 2, VEGF and MVD were studied by immunohistochemical method. RESULTS:The positive expression rate of COX 2 was 60 5% in gastric carcinoma tissue. It was significantly higher than that in control group. Overexpression of COX 2 in gastric carcinoma was related to its lymph node metastasis but it did not correlate with tumor size,depth of invasion and differentiation degree, P >0 05.The expression conformation rate of COX 2 and VEGF in gastric carcer was 53 5%. There was a positive correlation between expression of COX 2 and VEGF, P <0 01. The MVD values in COX 2 positive(22 08±3 69)and VEGF positive(22 12±3 50)groups was significantly higher than those in COX 2 negative and VEGF negative groups (17 68±3 50,16 15±3 23,respectively, P <0 05), and the MVD value (22 86±3 40, P <0 05) in both COX 2 positive and VEGF positive group was significantly higher than those in the other subgroups.CONCLUSIONS:The COX 2 protein detection,VEGF expression can be considered as biological indicators of malignant potential in gastric cancer. The COX 2 and VEGF expressions, both contributing to the tumor neovascularization, may be helpful in the understanding of intra tumor angiogenetic mechanism in the future.