一氧化氮——NF-κB信号通路在人初始T细胞向Th1/Th2分化中的作用

Role of nitric oxideNF——κB signaling pathway in differentiation of hu man nave Tlymphocytes into Th1/Th2 cells

目的 :探讨一氧化氮 (NO)核因子κB(NF κB)信号通路在人幼稚T细胞向 1型辅助性T细胞 (Th1) /2型辅助性T细胞(Th2 )分化过程中的作用。方法 :分离人脐血幼稚T细胞 ,分别加入不同浓度的NO供体硝普钠 (SNP)、NO合成抑制剂左旋精氨酸甲基酯 (NAME)和NF κB抑制剂吡咯二硫氨基甲酸酯(PDTC) ,通过流式细胞术检测细胞内IFN γIL 4的表达 ,了解幼稚T细胞的分化。结果 :在不同浓度的SNP和NAME和PDTC作用下 ,表达IFN γ(即Th1)或IL 4(即Th2 )T细胞的百分率与对照组相比较均无显著差异 (P >0 .0 5)。结论 :NONF κB信号通路对人幼稚T细胞向Th1和Th2细胞的分化均无显著影响。

AIM: To explore the role of nitric oxide(NO)NF-κB signaling p athway in the differentiation of human nave T lymphocytes into Th1/Th2 cells. METHODS: Human nave T lymphocytes were isolated from umbilic al blood. Various concentrations of NO donor sodium nitroprusside (SNP), NO inhi bitor NAME and NF-κB inhibitor PDTC were added to the culture medium to induce the differentiation of nave T cells towards Th1/Th2 cells. The expressions of intracellular cytokine IFN-γ and IL-4 were detected by flow cy tometry. RESULTS: The treatment of SNP, NAME and PDTC made no difference on the percentage of cells expressing IFN-γ(Th1) or IL- 4(Th2) in comparison with that of the control group (P<0.05). C ONCLUSION: Signaling pathway of NONF-κB had no effect on diff erentiation of human nave T lymphocytes into Th1 and Th2 cells. The role of NO NF-κB signaling pathway in the regulation of types 1 and 2 cytokines may occur mainly at the level of mature Th cells.