瘦素及瘦素长受体在多囊卵巢综合征患者子宫内膜的表达

Expression of leptin and long form of leptin receptors messenger ribonucleic acid and protein in endometrium of patients with polycystic ovary syndrome

目的 探讨瘦素 (leptin)和瘦素长受体 (Ob RL)mRNA及蛋白在多囊卵巢综合征(PCOS)患者着床期子宫内膜的表达及意义。方法 应用免疫组织化学技术及原位杂交技术 ,分别对15例正常妇女 (对照组 )、14例无排卵PCOS患者 (PCOS组 )促排卵治疗后着床期子宫内膜的leptin、Ob RL mRNA及蛋白进行测定。结果 对照组中 ,子宫内膜 10例呈分泌期中期改变 ,5例呈分泌期早期改变。PCOS组中 ,子宫内膜 3例呈分泌期中期改变 ,6例呈分泌期早期改变 ,1例呈增殖期改变 ,4例呈增殖期并分泌期早期改变 ;子宫内膜发育不良发生率 79% (11/ 14 ) ,与对照组比较 ,差异有显著性 (P <0 .0 5 )。Ob RL mRNA和蛋白在PCOS患者子宫内膜腺体呈阴性到强阳性表达 ,但多呈弱阳性和阳性表达 ,对照组则呈弱阳性到强阳性表达 ,两组比较 ,差异有显著性 (P <0 .0 5 ) ;leptinmRNA和蛋白在PCOS患者子宫内膜的表达与对照组相似 ,两组比较 ,差异无显著性 (P >0 .0 5 )。结论 促排卵周期中 ,Ob RL 在PCOS患者着床期子宫内膜腺体表达减弱 。

Objective To study the expression of leptin and long form of leptin receptors (Ob R L) messenger ribonucleic acid (mRNA) and protein in endometrium during mid luteal phase of patients with polycystic ovary syndrome (PCOS) Methods The expession of leptin and Ob R L mRNA in endometrium during mid luteal phase from 15 normal controls and 14 PCOS related anovulatory patients who underwent ovulation induction therapy were studied by in situ hybridization, and levels of leptin and Ob R L protein were investigated by immunohistochemical methods Results The expression of Ob R L mRNA and protein in endometrial glands of most patients with PCOS were weak or moderate positive,whereas the levels of Ob R L mRNA and protein were moderate or strong positive in those of the controls,there was significant difference between the two groups; leptin mRNA and protein expression in enodmetrium of patients with PCOS and controls were not significantly different between the groups Conclusion The expression of Ob R L decreased in endometrium of mid luteal phase in patients with PCOS during induced ovulation,which may be related to low pregnancy rate of patients with PCOS.