黄素化颗粒细胞端粒酶的表达及与卵巢功能关系的初步研究

Expression of telomerase in human ovarian luteinized granulosa cells and its relationship to ovarian function

目的 了解端粒酶在人卵巢黄素化颗粒细胞的表达 ,及与卵巢生殖功能的关系。方法 收集 2 2例行体外受精 胚胎移植或卵胞浆单精子显微注射患者的卵巢黄素化颗粒细胞 ,采用原位杂交法及端粒末端重复序列扩增法 ,分别检测人端粒酶催化亚单位 (hTERT)mRNA及端粒酶活性。结果 黄素化颗粒细胞普遍表达hTERTmRNA ,但其表达强弱不一。 2 2份卵巢黄素化颗粒细胞标本中 ,16份 (73% )有端粒酶活性 ,与血清基础促卵泡激素水平呈负相关 (P <0 .0 1) ,且随年龄增长呈下降趋势。结论 端粒酶可能对维持卵巢功能起重要作用 。

Objective To detect the expression of telomerase catalytic subunit (TERT),telomerase in human ovarian luteinized granulosa cells and to investigate the relationship between telomerase expression and the ovarian fecundity Methods Ovarian luteinized granulosa cells were recovered from 22 women with regular menses who underwent in vitro fertilization/ intracytoplasmic sperm injection programme.We carried out in situ hybridization histochemistry on luteinized granulosa cells to detect TERT mRNA expression,and telomeric repeat amplification protocol to detect telomerase activity Results TERT mRNA were present in luteinized granulosa cells.The expression of TERT mRNA and telomerase activity in ovarian luteinized granulosa cells decreased with increase of age,basal serum follicle stimulating hormone levels Conclusions These results suggest that telomerase may play an active role in ovarian function.A reduced telomerase activity of granulosa cells may be one of the important mechanisms involved in decreased ovarian function in women.