ATP敏感性钾离子(K_(ATP))通道的mRNA在大鼠颈动脉体的表达(英文)

EXPRESSION OF mRNA FOR ATP-SENSITIVE POTASSIUM CHANNELS IN RAT CAROTID BODY

目的 探讨因缺氧引起颈动脉体K+ 流减少而导致颈动脉体神经活性增加的分子学机制。 方法 利用逆转录聚合酶链反应 (RT PCR)评价大鼠颈动脉体的ATP敏感性钾离子 (KATP)通道的mRNA表达。 结果 在大鼠颈动脉体中内向整流性钾离子通道亚家族Kir6 .1的mRNA是存在的 ,而Kir6 .2和磺酰脲受体 (SUR1andSUR2 )则未见。 结论 Kir6 .

Objective To understand the molecular mechanism by which hypoxia leads to an increase in sensory nerve activity by inhibiting K + current,the molecular correlates for the K + current have to be identified. Methods The expression of mRNA for ATP sensitive potassium (K ATP )channels was studied in rat carotid body using reverse transcription polymerase chain reaction (RT PCR). Results In rat carotid body,transcript for inwardly rectifying K + channel subfamily Kir6.1 was present,whereas transcripts for Kir 6.2,sulfonylurea receptors(SUR1 and SUR2)were not found.Conclusion\ Rat carotid body expresses the Kir6.1,and suggests that K ATP channels may play a role in the carotid body during hypoxia.\;[