金雀黄素抑制人乳腺癌细胞系体外增殖作用机理的实验研究

EXPERIMENTAL STUDY ON MECHANISM OF INHIBITING PROLIFEROUS EFFECT ON HUMAN BREAST CANCER CELL LINES BY GENISTEIN in vitro

目的 研究金雀黄素抑制人乳腺癌细胞株体外增殖作用的机理。 方法 选用 2种不同的人乳腺癌细胞株MCF 7和MDA MB 2 31体外培养 ,MTT法检测细胞增殖作用 ,Giemsa染色观察细胞形态学改变 ,流式细胞仪检测细胞周期及凋亡率 ,同时进行了原位细胞凋亡的检测。 结果 金雀黄素对MCF 7细胞和MDA MB 2 31细胞体外生长具有明显的抑制作用。Giemsa染色显示 ,金雀黄素处理的MCF 7细胞呈明显的凋亡形态改变 ,细胞固缩、发泡 ,染色质凝集呈块状 ,并沿核周分布。流式细胞仪检测在G1 峰前可见凋亡峰 ,且随用药时间的延长 ,凋亡比率递增。而金雀黄素处理的MDA MB 2 31细胞未见明显的凋亡形态改变和凋亡峰的出现 ,细胞周期明显地阻滞于G2 ～M期。 结论 金雀黄素通过诱导细胞凋亡或阻滞细胞于G2 M期 ,从而抑制乳腺癌细胞的体外增殖 。

Objective To study the mechanisms of inhibiting proliferous effect of human breast cancer cell line treated with genistein in vitro. Methods Two human breast cancer cell lines (MCF 7 and MDA MB 231) were cultured in vitro , proliferation of them was measured with MTT method, cell morphology was observed with Giemsa stain, cell cycle and apoptosis rate were measured with flow cytometry and apoptosis was detected in situ. Results Genistein markedly inhibited proliferation of MCF 7 and MDA MB 231 cell line. Typical image of apoptosis was observed in MCF 7 cell line treated with genistein in Giemsa stain:cell condensation, blebs formation on cell surface and pieces of condensed chromatin were scattered along nuclear envelope. The results detected with flow cytometry showed G 1 subpeak before G 1 phase peak (apoptosis peak). Apoptosis rate increased with time of genistein treatment. However, MDA MB 231 cell line treated with genistein did not show apoptosis and apoptosis peak, and quantity of cells at G 2 M phase obviously increased.Conclusion Genistein inhibites proliferation of human breast cancer cell line in vitro through inducing apoptosis or arresting cell at G 2 M phase.\;[