肝门部胆管癌中MMP-2/TIMP-2比值半定量研究的临床意义

Clinical significance in the relationship between the ratio of MMP - 2/TIMP - 2 of hilar cholangiocarcinoma

目的 探讨MMP-2和TIMP-2在肝门部胆管癌浸润、转移过程中的作用及其与预后的关系,为判断肿瘤恶性程度、浸润转移、预后风险及提高临床治疗效果提供实验依据。方法 采用免疫组化法结合计算机图象分析软件技术检测肝门部胆管癌中MMP-2和TIMP-2的表达情况,将临床结合相关病理参数进行分析及统计学处理。结果 MMP-2的表达肝内有浸润组高于肝内无浸润组,TIMP-2的表达相反。图象定量分析结果显示:二者阳性染色强度指数比值与肿瘤临床分型、局部淋巴结有无转移、有无肝内浸润转移、肿瘤组织分化程度和生存期有关(P<0.05);与肿瘤组织学类型无关(P>0.05)。结论 MMP-2有促进肿瘤浸润转移功能,TIMP-2具有抗肿瘤浸润转移功能,临床上检测MMP-2/TIMP-2比值有助于肝门部胆管癌生物学行为的判断和预后风险的估计。

Objective To evaluate the roles of MMP - 2 and its inhibitor TTMP - 2 in tumor invasion or as a prognostic factor in patients with human hilar cholangiocarcinoma. Methods The expression of MMP - 2 and TTMP - 2 were investigated in patients. Specimens obtained from 50 patients with human hilar cholangiocarcinoma were used for analysis. Expression of MMP - 2 and TIMP - 2 was examined by immunohistochemical analysis. Image analysis with Image - Pro Plus Analysis Software was used to semiquantitatively determine the ratio of MMP - 2/ TIMP - 2. Results The expression of MMP - 2 and TIMP - 2 were strongly associated with tumor hepatic invasion in patients with H - CC. The ratio of MMP - 2 to TIMP - 2 expression were significantly correlated with some pathologic factors in patients with H - CC. Conclusions MMP - 2 has an essential role in tumor invasion and metastasis , while TTMP - 2 is shown to strongly inhibit cancer invasion and metastasis. The ratio of MMP - 2/TIMP -2 may be a prognostic indicator for patients with human hilar cholangiocarcinoma.