辽宁与四川两省嗜人按蚊细胞遗传学研究

Studies on cytogenetics of Anopheles anthropophagus between Lianng strain and Sichuan strain

目的 确定辽宁省可疑按蚊与嗜人按蚊的遗传关系。方法 以人工诱导法和自然交配法对辽宁省可疑嗜人按蚊与四川省嗜人按蚊进行杂交实验 ,观察杂交卵的孵化率、幼虫生长情况、化蛹率、羽化率 ,判定辽宁省可疑嗜人按蚊与四川省嗜人按蚊是否存在生殖隔离 ;按Kanda法制作幼虫唾腺染色体 ,观察杂交F1代幼虫唾腺染色体联会情况。结果 两地按蚊人工诱导杂交卵的孵化率为 72 36 % ,化蛹率 70 13% ,羽化率 92 5 4 % ;自然交配孵化率、化蛹率和羽化率分别为 87 72 %、70 99%和 95 0 9%。杂交F1代与亲本回交和自交 ,杂交F2 代均能正常发育。杂交F1代幼虫唾腺染色体完全联会。结论 两地嗜人按蚊不存在生殖隔离 ,辽宁省可疑按蚊确系嗜人按蚊无疑。

Aim To define the genetic relation between the Lianng dubious species and A anthropophagus Methods The cross experiments were made by the means of induceing copulation and natural mating between Lianng undefined strain and Sichuan strain of A anthropophagus The reproductive isolation was confirmed in the hatching rate,the growing of larvae,pupate rate and emergencing rate in F 1 hybrids The salivary chromosomes were preparated in the early four instar by the methods of described by Kanda and the chromosomes synapsis were observed in F 1 hybrids Result The hatch rate was 72 36%,the pupation rate was 70 13% and the emergence rate was 92 54% by the means of induced population,and 87 72%,70 99% and 95 09% in the natural mating respectively The hybrids F 2 were growing well from the back cross or selfed of the hybrids F 1 The salivary chromosome was complete synapsis in F1 hybrids Conclusion There is no reproductive isolation between Lianng strain and Sichuan strain,The dubious strain in Lianng Province has been defined to be A anthropophagus