ras癌基因产物P21免疫组化检测与Ki-ras转录表达在人肺癌中的比较研究

COMPARATIVE STUDY OF ras ONCOGENE P21 PRODUCT AND Ki-ras ONCOGENE mDNA EXPRESSION IN HUMAN LUNG CARCINOMA

对23例人肺癌组织进行P21的免疫组化检测和总RNA的Ki-ras mRNA斑点杂交。分别得到肺癌组织的P21蛋白表达和Ki-ras mRNA转录表达水平的半定量值,并对这一对半定量值进行统计学相关性分析,证实两者呈正相关(r′=0.60 P<0.005)。这支持利用免疫组化技术检测P21表达水平可以反映ras癌基因mRNA转录表达情况,值得进一步积累经验和用于有关基础研究和临床。

23 cases of human lung carcinoma were studied by immunohistochemical staining with anti-P_(21) monoclonal antibody and Ki-ras mRNA dot blot method. According to semi-quantitative results, the statistics demonstrated significant rank correlation between both groups (r'=0.60, P<0.005). It was suggested that immunohistochemical detection of ras oncogene P_(21) antibody may be used to evalute the quantity of ras oncogene mRNA in lung carcinoma.