摘要
目的 探讨酶联免疫吸附试验(ELISA)检测泌尿生殖道分泌物及皮损中单纯疱疹病毒(HSV)抗原在临床中的应用价值。方法 用聚合酶链反应(PCR)和ELISA方法检测泌尿生殖道及皮损中HSV DNA和HSV抗原,并对两种方法的检测结果进行比较。结果 118例标本中,PCR检出41例阳性,ELISA 42例阳性。在118例标本中,111例两种方法结果相符,7例不相符合:PCR阳性的41例小,ELISA阳忭38例(敏感性92.68％);PCR阴性的77例标本中,ELISA阳性4例(特异性94.81％)。结论 ELISA检测HSV抗原的方法可直接检测出泌尿生殖道及皮损中HSV病原体,从而为生殖器疱疹诊断提供准确的实验依据,其敏感性、特异性高,方便,快速,值得临床推广应用。
Objectives To evaluate the use of enzyme linked immunosorbent assay (KLISA) method in the detection herpes simplex virus (HSV) antigen in genitourinary tract and dermal lesions herpes. Methods The specimens were tested with ELISA and polymerase chain reaction (PCR) in 118 patients. The results of 2 test methods was compared. Results Among 118 patients, HSV was detected in 42 by ELISA and 41 by PCR. 111 of 118 specimens showed the same results by both tests. In comparison with PCR, the relative sensitivities, specificities of ELISA were 92.68% and 94.81% respectively. Conclusion ELISA could directly detect HSV pathogen in genitourinary tract and dermal lesions and be simple, rapid, high sensitive and specific for the diagnosis of genital herpes.
出处
《中国中西医结合皮肤性病学杂志》
CAS
2004年第1期29-30,共2页
Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine
