大鼠同种异体肢体移植中T淋巴细胞亚群的变化及其意义

Changes and significance of T lymphocyte subsets in rat limb allotransplantation

目的 建立大鼠同种异体肢体移植模型 ,研究肢体移植中T细胞亚群的变化及其与急性排斥反应的关系。方法 将 3 1只SD雄性大鼠随机分成两组 ,其中对照组 14只为Wistar→SD肢体移植术后不用药组 ,实验组 17只为FK5 0 6( 4mg/kg体重 ) +RS 614 43 ( 3 0mg/kg体重 )联合免疫抑制治疗组 ,观察各组移植术后排斥反应 ;用免疫荧光染色流式细胞仪检测各组手术前后T细胞亚群。结果 术后第 3天 ,CD4/CD8比值显著增高 ,移植肢体开始出现肿胀、皮肤红斑等表现 ;CD4/CD8比值增高与排斥反应程度呈正相关 ,而用药组术后 ,CD4/CD8较术前无明显变化 ,未出现排斥反应。结论 T细胞亚群中CD4/CD8比值的变化与肢体移植急性排斥反应的发生、强烈程度有密切关系 ,可作为监测异体肢体移植急性排斥反应的免疫学指标。

Objective To explore the relation between the acute rejection and T lymphocyte subsets through the model of limb allotransplantation in rats.Methods 31 male SD rats were randomly divided into two groups: control group ( n =14) and experimental group ( n =17).In control group,Wistar to SD limb allotransplantation was performed without using drugs after operation.In experimental group,the recipients were immunosuppressed with FK506 (4 mg/kg) in combination with RS 61443 (30 mg/kg).The rejection time and the histologic degree of skin rejection of a limb allograft were investigated.T lymphocyte subsets before and after operation were also assayed by using immunofluorescence stain and flow cytometry,and the relationship between the changes in T lymphocyte subsets and the rejection time or the degree of rejection was observed.Results In the control group,the ratio of CD 4/CD 8 was obviously increased on the 3rd postoperative day and was positively related to the degree of rejection.In the experimental group,there was no significant difference in the ratio of CD 4/CD 8 before and after operation,and no rejection occurred.Conclusion The ratio of CD 4/CD 8 was closely related to the occurrence of rejection in limb allografts and its degree,so it can act as an immunologic indicator to monitor acute rejection of limb allografts.